Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACLZ0G_RS21705 Genome accession   NZ_CP178552
Coordinates   4426567..4428000 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain WWCOL-236     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4421567..4433000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLZ0G_RS21675 (ACLZ0G_21675) yrbG 4422125..4423102 (+) 978 WP_022646167.1 calcium/sodium antiporter -
  ACLZ0G_RS21680 (ACLZ0G_21680) kdsD 4423116..4424102 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACLZ0G_RS21685 (ACLZ0G_21685) kdsC 4424123..4424689 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACLZ0G_RS21690 (ACLZ0G_21690) lptC 4424686..4425261 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACLZ0G_RS21695 (ACLZ0G_21695) lptA 4425230..4425787 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACLZ0G_RS21700 (ACLZ0G_21700) lptB 4425794..4426519 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACLZ0G_RS21705 (ACLZ0G_21705) rpoN/rpoN1 4426567..4428000 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACLZ0G_RS21710 (ACLZ0G_21710) hpf 4428023..4428310 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACLZ0G_RS21715 (ACLZ0G_21715) ptsN 4428428..4428919 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACLZ0G_RS21720 (ACLZ0G_21720) rapZ 4428965..4429819 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ACLZ0G_RS21725 (ACLZ0G_21725) npr 4429816..4430088 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACLZ0G_RS21730 (ACLZ0G_21730) yrbL 4430301..4430933 (+) 633 WP_000620399.1 PhoP regulatory network protein YrbL -
  ACLZ0G_RS21735 (ACLZ0G_21735) mtgA 4430930..4431658 (-) 729 WP_000047098.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACLZ0G_RS21740 (ACLZ0G_21740) elbB 4431655..4432308 (-) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=972450 ACLZ0G_RS21705 WP_000809051.1 4426567..4428000(+) (rpoN/rpoN1) [Escherichia coli strain WWCOL-236]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=972450 ACLZ0G_RS21705 WP_000809051.1 4426567..4428000(+) (rpoN/rpoN1) [Escherichia coli strain WWCOL-236]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACTGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465