Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACLZ2J_RS13735 Genome accession   NZ_CP178514
Coordinates   2619947..2620357 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain BS18-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2614947..2625357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLZ2J_RS13705 - 2615034..2615174 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ACLZ2J_RS13710 yqeF 2615480..2616211 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ACLZ2J_RS13715 cwlH 2616463..2617215 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ACLZ2J_RS13720 yqeD 2617402..2618028 (+) 627 WP_024572279.1 TVP38/TMEM64 family protein -
  ACLZ2J_RS13725 gnd 2618047..2618940 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACLZ2J_RS13730 yqeB 2619192..2619914 (+) 723 WP_010886572.1 hypothetical protein -
  ACLZ2J_RS13735 nucA/comI 2619947..2620357 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ACLZ2J_RS13740 - 2620553..2620903 (+) 351 Protein_2659 sigma-70 family RNA polymerase sigma factor -
  ACLZ2J_RS13745 spoIVCA 2620931..2622391 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  ACLZ2J_RS13750 - 2622349..2622527 (-) 179 Protein_2661 hypothetical protein -
  ACLZ2J_RS13755 arsC 2622882..2623301 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ACLZ2J_RS13760 acr3 2623313..2624353 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  ACLZ2J_RS13765 arsK 2624376..2624816 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ACLZ2J_RS13770 arsR 2624877..2625194 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=972162 ACLZ2J_RS13735 WP_009967785.1 2619947..2620357(-) (nucA/comI) [Bacillus subtilis strain BS18-1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=972162 ACLZ2J_RS13735 WP_009967785.1 2619947..2620357(-) (nucA/comI) [Bacillus subtilis strain BS18-1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment