Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   WOC63_RS01745 Genome accession   NZ_CP150765
Coordinates   361332..362960 (-) Length   542 a.a.
NCBI ID   WP_001186608.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM20963     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 345932..393801 361332..362960 within 0
SCCmec 354567..364331 361332..362960 within 0


Gene organization within MGE regions


Location: 345932..393801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC63_RS01665 (WOC63_01665) - 345932..346606 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  WOC63_RS01670 (WOC63_01670) - 346697..347680 (-) 984 Protein_331 protein rep -
  WOC63_RS01675 (WOC63_01675) mobV 347925..349186 (-) 1262 Protein_332 MobV family relaxase -
  WOC63_RS01680 (WOC63_01680) bleO 349693..350097 (-) 405 WP_001242578.1 bleomycin binding protein -
  WOC63_RS01685 (WOC63_01685) aadD1 350314..351084 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  WOC63_RS01690 (WOC63_01690) - 351277..351951 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  WOC63_RS01695 (WOC63_01695) - 352035..352376 (+) 342 WP_001797647.1 hydroxymethylglutaryl-CoA synthase -
  WOC63_RS01700 (WOC63_01700) - 353253..353996 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  WOC63_RS01705 (WOC63_01705) - 354093..354521 (+) 429 WP_000872606.1 MaoC family dehydratase -
  WOC63_RS01710 (WOC63_01710) mecA 354567..356576 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  WOC63_RS01715 (WOC63_01715) - 356673..357659 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  WOC63_RS01720 (WOC63_01720) - 357649..357891 (+) 243 Protein_341 type I restriction enzyme endonuclease domain-containing protein -
  WOC63_RS01725 (WOC63_01725) - 357882..359492 (-) 1611 Protein_342 IS1182 family transposase -
  WOC63_RS01730 (WOC63_01730) - 359541..360047 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  WOC63_RS01735 (WOC63_01735) - 360062..360373 (-) 312 WP_000700851.1 DUF960 family protein -
  WOC63_RS01740 (WOC63_01740) - 360460..360810 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  WOC63_RS01745 (WOC63_01745) ccrB 361332..362960 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  WOC63_RS01750 (WOC63_01750) ccrA 362982..364331 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  WOC63_RS01755 (WOC63_01755) cch1 364565..366358 (-) 1794 WP_001077072.1 cassette chromosome replicative helicase -
  WOC63_RS01760 (WOC63_01760) ssb 366358..366666 (-) 309 WP_000660731.1 cassette chromosome ssDNA-binding protein -
  WOC63_RS01765 (WOC63_01765) - 366931..368484 (-) 1554 WP_000477478.1 hypothetical protein -
  WOC63_RS01770 (WOC63_01770) - 368682..368882 (-) 201 Protein_351 arsenate reductase -
  WOC63_RS01775 (WOC63_01775) - 368860..369124 (-) 265 Protein_352 ArsR/SmtB family transcription factor -
  WOC63_RS01780 (WOC63_01780) - 369255..373976 (+) 4722 WP_368409789.1 Rib/alpha-like domain-containing protein -
  WOC63_RS01785 (WOC63_01785) - 374666..374800 (-) 135 WP_001790628.1 hypothetical protein -
  WOC63_RS01790 (WOC63_01790) - 374764..374856 (+) 93 WP_001790000.1 hypothetical protein -
  WOC63_RS01795 (WOC63_01795) - 375003..376748 (+) 1746 WP_000700190.1 DUF6119 family protein -
  WOC63_RS01800 (WOC63_01800) - 376842..377300 (-) 459 Protein_357 tRNA-dihydrouridine synthase -
  WOC63_RS01805 (WOC63_01805) - 377310..377411 (-) 102 WP_001791873.1 hypothetical protein -
  WOC63_RS01810 (WOC63_01810) - 377509..378264 (-) 756 WP_000355845.1 sulfite exporter TauE/SafE family protein -
  WOC63_RS01815 (WOC63_01815) cstR 378264..378524 (-) 261 WP_001105087.1 persulfide-sensing transcriptional repressor CstR -
  WOC63_RS01820 (WOC63_01820) cstA 378661..379728 (+) 1068 WP_000816129.1 persulfide response sulfurtransferase CstA -
  WOC63_RS01825 (WOC63_01825) cstB 379759..381093 (+) 1335 WP_000465472.1 persulfide dioxygenase-sulfurtransferase CstB -
  WOC63_RS01830 (WOC63_01830) sqr 381112..382305 (+) 1194 WP_001033018.1 type II sulfide:quinone oxidoreductase Sqr -
  WOC63_RS01835 (WOC63_01835) - 382572..382786 (-) 215 Protein_364 universal stress protein -
  WOC63_RS01840 (WOC63_01840) - 382972..383958 (-) 987 WP_000662031.1 tRNA-dihydrouridine synthase -
  WOC63_RS01845 (WOC63_01845) - 384267..384470 (+) 204 WP_000398679.1 DUF6007 family protein -
  WOC63_RS01850 (WOC63_01850) - 384519..384815 (+) 297 WP_000201640.1 TfoX/Sxy family protein -
  WOC63_RS01855 (WOC63_01855) - 384887..386107 (-) 1221 WP_000130764.1 MFS transporter -
  WOC63_RS01860 (WOC63_01860) - 386100..386651 (-) 552 WP_000627022.1 flavodoxin family protein -
  WOC63_RS01865 (WOC63_01865) - 386764..387624 (+) 861 WP_001052510.1 LysR family transcriptional regulator -
  WOC63_RS01870 (WOC63_01870) - 387979..388512 (-) 534 WP_368409790.1 DUF4865 family protein -
  WOC63_RS01875 (WOC63_01875) - 388603..389346 (+) 744 WP_001793250.1 LysR family transcriptional regulator -
  WOC63_RS01880 (WOC63_01880) - 389780..390382 (+) 603 WP_000908952.1 hypothetical protein -
  WOC63_RS01885 (WOC63_01885) - 390649..393801 (+) 3153 WP_000080559.1 ATP-binding protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62654.83 Da        Isoelectric Point: 9.7970

>NTDB_id=972121 WOC63_RS01745 WP_001186608.1 361332..362960(-) (ccrB) [Staphylococcus aureus strain TUM20963]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=972121 WOC63_RS01745 WP_001186608.1 361332..362960(-) (ccrB) [Staphylococcus aureus strain TUM20963]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAACGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTGCTATGCCAAAGGACATGGCTATCGTAAAATTGCCAATGCATTGAATCACAAAGGATATGTCACTAAAA
AGGGGAAACCTTTCAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAACACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(180-280)

Resolvase, N terminal domain (Resolvase)

(11-156)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.893

100

0.989

Multiple sequence alignment