Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACL09D_RS08225 Genome accession   NZ_CP178401
Coordinates   1672391..1673824 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain N1286     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1667391..1678824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL09D_RS08195 (ACL09D_08195) yrbG 1667949..1668926 (+) 978 WP_000922872.1 calcium/sodium antiporter -
  ACL09D_RS08200 (ACL09D_08200) kdsD 1668940..1669926 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACL09D_RS08205 (ACL09D_08205) kdsC 1669947..1670513 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACL09D_RS08210 (ACL09D_08210) lptC 1670510..1671085 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACL09D_RS08215 (ACL09D_08215) lptA 1671054..1671611 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACL09D_RS08220 (ACL09D_08220) lptB 1671618..1672343 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACL09D_RS08225 (ACL09D_08225) rpoN/rpoN1 1672391..1673824 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACL09D_RS08230 (ACL09D_08230) hpf 1673847..1674134 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACL09D_RS08235 (ACL09D_08235) ptsN 1674252..1674743 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACL09D_RS08240 (ACL09D_08240) rapZ 1674789..1675643 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ACL09D_RS08245 (ACL09D_08245) npr 1675640..1675912 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACL09D_RS08250 (ACL09D_08250) yrbL 1676125..1676757 (+) 633 WP_000620387.1 PhoP regulatory network protein YrbL -
  ACL09D_RS08255 (ACL09D_08255) mtgA 1676754..1677482 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACL09D_RS08260 (ACL09D_08260) elbB 1677479..1678132 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=971771 ACL09D_RS08225 WP_000809051.1 1672391..1673824(+) (rpoN/rpoN1) [Escherichia coli strain N1286]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=971771 ACL09D_RS08225 WP_000809051.1 1672391..1673824(+) (rpoN/rpoN1) [Escherichia coli strain N1286]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465