Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   WOC43_RS04010 Genome accession   NZ_CP150749
Coordinates   832192..833541 (+) Length   449 a.a.
NCBI ID   WP_117219606.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22699     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 814451..849966 832192..833541 within 0
SCCmec 832192..841909 832192..833541 within 0


Gene organization within MGE regions


Location: 814451..849966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC43_RS03950 (WOC43_03955) - 815309..816361 (+) 1053 WP_001835016.1 Mur ligase family protein -
  WOC43_RS03955 (WOC43_03960) - 816387..817361 (+) 975 WP_015445693.1 hypothetical protein -
  WOC43_RS03960 (WOC43_03965) - 817384..818895 (+) 1512 WP_000640645.1 glycosyltransferase -
  WOC43_RS03965 (WOC43_03970) - 819055..820671 (-) 1617 WP_000393940.1 hypothetical protein -
  WOC43_RS03970 (WOC43_03975) - 820795..824328 (-) 3534 WP_368410270.1 E domain-containing protein -
  WOC43_RS03975 (WOC43_03980) - 824543..825301 (-) 759 WP_000623680.1 sulfite exporter TauE/SafE family protein -
  WOC43_RS03980 (WOC43_03985) cstR 825298..825558 (-) 261 WP_000456194.1 persulfide-sensing transcriptional repressor CstR -
  WOC43_RS03985 (WOC43_03990) - 825697..826764 (+) 1068 WP_000085031.1 DsrE/DsrF/DrsH-like family protein -
  WOC43_RS03990 (WOC43_03995) cstB 826783..828111 (+) 1329 WP_000465464.1 persulfide dioxygenase-sulfurtransferase CstB -
  WOC43_RS03995 (WOC43_04000) - 828191..829768 (-) 1578 WP_000271874.1 hypothetical protein -
  WOC43_RS04000 (WOC43_04005) ssb 829939..830235 (+) 297 WP_000120684.1 cassette chromosome ssDNA-binding protein -
  WOC43_RS04005 (WOC43_04010) cch1 830235..832004 (+) 1770 WP_000017983.1 cassette chromosome replicative helicase -
  WOC43_RS04010 (WOC43_04015) ccrA 832192..833541 (+) 1350 WP_117219606.1 cassette chromosome recombinase CcrA Machinery gene
  WOC43_RS04015 (WOC43_04020) ccrB 833563..835187 (+) 1625 Protein_780 cassette chromosome recombinase CcrB -
  WOC43_RS04020 (WOC43_04025) - 835653..836003 (+) 351 WP_000859157.1 SAUGI family uracil-DNA glycosylase inhibitor -
  WOC43_RS04025 (WOC43_04030) - 836090..836401 (+) 312 WP_001024803.1 DUF960 family protein -
  WOC43_RS04030 (WOC43_04035) - 836416..836922 (+) 507 WP_001092195.1 DUF1643 domain-containing protein -
  WOC43_RS04035 (WOC43_04040) - 836974..838575 (+) 1602 Protein_784 IS1182 family transposase -
  WOC43_RS04040 (WOC43_04045) - 838585..838827 (-) 243 Protein_785 type I restriction enzyme endonuclease domain-containing protein -
  WOC43_RS04045 (WOC43_04050) - 838817..839803 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  WOC43_RS04050 (WOC43_04055) mecA 839900..841909 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  WOC43_RS04055 (WOC43_04060) - 841955..842383 (-) 429 WP_000872606.1 MaoC family dehydratase -
  WOC43_RS04060 (WOC43_04065) - 842480..843223 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  WOC43_RS04065 (WOC43_04070) - 844100..844489 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  WOC43_RS04070 (WOC43_04075) - 844525..845199 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  WOC43_RS04075 (WOC43_04080) mobV 845337..846599 (-) 1263 WP_000119405.1 MobV family relaxase -
  WOC43_RS04080 (WOC43_04085) bleO 847106..847510 (-) 405 WP_001242578.1 bleomycin binding protein -
  WOC43_RS04085 (WOC43_04090) aadD1 847727..848497 (-) 771 WP_001795128.1 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia -
  WOC43_RS04090 (WOC43_04095) - 848657..849640 (-) 984 WP_000336291.1 protein rep -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52581.35 Da        Isoelectric Point: 9.9370

>NTDB_id=971736 WOC43_RS04010 WP_117219606.1 832192..833541(+) (ccrA) [Staphylococcus aureus strain TUM22699]
MKQAIGYLRQSTTKQQSLAAQKQTIEALAKKHNIQYITFYSDKQSGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKAKQGKMITTHAPFGYHYH
NGTFTIDTVKAPTVKAVFNYYLQGYGYKKIAQYLEADDKFINRKPYQVRNIILNPNYCGRVINQYGQYENMFPAIVSTTI
YEEAQVTRTQKPVKRKPSENQLKQKIKCPYCDSTLTNMTIRKKHHTLRYYVCPQNMNASRFVCEFKGINAQELETSVLAT
CQDFFQNQQLYSKINHTIQQRLKRQRDIETKTTLNHEQLIEKLAQDKIDAETFREQTQSLRQQSKPISSISTYQIRKAFQ
NIIQQRFTLNMLYPYIDEINNSKNKSLAGIYFKNEPLNIVKQTMELSIV

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=971736 WOC43_RS04010 WP_117219606.1 832192..833541(+) (ccrA) [Staphylococcus aureus strain TUM22699]
ATGAAACAAGCAATAGGTTACTTACGACAGAGCACTACAAAGCAACAATCCTTAGCAGCACAAAAACAAACCATCGAGGC
ATTAGCCAAAAAACATAATATTCAATACATTACCTTTTATAGCGATAAGCAATCAGGACGCACTGATAAGCGGAACGGTT
ACCAACAAATTACTGAACTGATTCAACAAGGACAATGTGATGTATTATGTTGTTACAGATTAAACCGACTTCATCGCAAT
CTTAAAAATGCATTAAAACTCATGAAATTGTGTCAAAAATACCATGTCCATATCTTAAGCGTTCATGATGGCTATTTTGA
TATGGATAAAGCATTCGATCGGCTCAAACTCAATATTTTCATCAGCTTGGCCGAACTAGAATCTGATAATATAGGCGAAC
AAGTCAAAAATGGAATCAAAGAAAAAGCGAAACAAGGTAAAATGATTACAACACATGCACCCTTTGGGTATCACTATCAT
AATGGTACTTTCACGATAGACACAGTAAAAGCACCAACAGTAAAAGCTGTGTTCAATTATTACCTTCAAGGTTATGGTTA
TAAAAAAATTGCGCAATACTTAGAAGCTGATGATAAATTCATTAATCGTAAGCCCTATCAAGTGCGTAATATTATCCTTA
ACCCTAATTACTGTGGCCGTGTTATCAATCAATACGGACAATATGAAAACATGTTCCCAGCTATTGTCAGTACAACGATA
TACGAAGAAGCTCAAGTTACCCGAACTCAGAAACCAGTAAAACGTAAGCCGTCAGAAAACCAACTCAAACAAAAAATCAA
ATGTCCTTATTGTGATTCAACACTAACTAATATGACCATTAGAAAAAAGCACCATACATTACGTTATTATGTTTGTCCTC
AAAATATGAATGCATCTCGTTTTGTCTGTGAATTCAAAGGGATCAACGCACAAGAATTAGAAACAAGTGTTTTAGCGACT
TGTCAGGACTTCTTTCAAAATCAACAGCTCTATTCAAAAATAAACCATACTATTCAACAACGACTCAAAAGACAAAGAGA
TATAGAAACTAAAACTACACTCAATCACGAGCAGCTGATAGAAAAATTAGCCCAAGACAAAATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAGCAATCAAAACCTATATCATCAATCAGTACGTATCAAATTCGAAAAGCTTTCCAA
AACATCATTCAACAACGTTTCACGCTAAACATGTTGTACCCCTATATTGATGAAATTAATAATTCTAAAAATAAAAGCCT
CGCTGGCATCTATTTCAAAAATGAACCACTGAATATTGTAAAGCAGACTATGGAATTATCAATTGTATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(170-249)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

99.777

100

0.998
  ccrA Staphylococcus aureus N315

74.61

100

0.746

Multiple sequence alignment