Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACLKPV_RS03880 Genome accession   NZ_CP178240
Coordinates   803428..804810 (+) Length   460 a.a.
NCBI ID   WP_001029687.1    Uniprot ID   P24554
Organism   Escherichia coli strain JW-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 798428..809810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKPV_RS03860 (ACLKPV_03860) yjjJ 799285..800616 (+) 1332 WP_001293115.1 type II toxin-antitoxin system HipA family toxin YjjJ -
  ACLKPV_RS03865 (ACLKPV_03865) lplA 800617..801633 (-) 1017 WP_000105884.1 lipoate--protein ligase LplA -
  ACLKPV_RS03870 (ACLKPV_03870) ytjB 801661..802305 (-) 645 WP_000124615.1 YtjB family periplasmic protein -
  ACLKPV_RS03875 (ACLKPV_03875) serB 802411..803379 (+) 969 WP_001132955.1 phosphoserine phosphatase -
  ACLKPV_RS03880 (ACLKPV_03880) radA/sms 803428..804810 (+) 1383 WP_001029687.1 DNA repair protein RadA Machinery gene
  ACLKPV_RS03885 (ACLKPV_03885) nadR 804831..806063 (+) 1233 WP_000093814.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACLKPV_RS03890 (ACLKPV_03890) - 806115..806600 (-) 486 Protein_762 helix-turn-helix domain-containing protein -
  ACLKPV_RS03895 (ACLKPV_03895) creA 806811..807284 (+) 474 WP_000875487.1 protein CreA -
  ACLKPV_RS03900 (ACLKPV_03900) creB 807297..807986 (+) 690 WP_001188654.1 two-component system response regulator CreB -
  ACLKPV_RS03905 (ACLKPV_03905) creC 807986..809410 (+) 1425 WP_001219577.1 two-component system sensor histidine kinase CreC -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49472.10 Da        Isoelectric Point: 7.1936

>NTDB_id=971074 ACLKPV_RS03880 WP_001029687.1 803428..804810(+) (radA/sms) [Escherichia coli strain JW-5]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPMVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=971074 ACLKPV_RS03880 WP_001029687.1 803428..804810(+) (radA/sms) [Escherichia coli strain JW-5]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P24554

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis NCTC 12261

43.86

99.13

0.435
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43

Multiple sequence alignment