Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V8V52_RS09215 Genome accession   NZ_CP150648
Coordinates   1762732..1763166 (+) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides strain 4BM1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1757732..1768166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8V52_RS09195 (V8V52_09195) - 1758510..1759673 (-) 1164 WP_002033816.1 aminotransferase A -
  V8V52_RS09200 (V8V52_09200) - 1759908..1760108 (-) 201 WP_000929276.1 hypothetical protein -
  V8V52_RS09205 (V8V52_09205) - 1760418..1761692 (+) 1275 WP_002014814.1 HAMP domain-containing sensor histidine kinase -
  V8V52_RS09210 (V8V52_09210) - 1761752..1762495 (-) 744 WP_002033817.1 DUF3967 domain-containing protein -
  V8V52_RS09215 (V8V52_09215) nucA/comI 1762732..1763166 (+) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  V8V52_RS09220 (V8V52_09220) comJ 1763206..1763595 (+) 390 WP_002088359.1 competence protein ComJ -
  V8V52_RS09225 (V8V52_09225) metE 1763636..1765924 (-) 2289 WP_002014819.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  V8V52_RS09230 (V8V52_09230) - 1766596..1767153 (-) 558 WP_000062071.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=970924 V8V52_RS09215 WP_002014817.1 1762732..1763166(+) (nucA/comI) [Bacillus mycoides strain 4BM1]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=970924 V8V52_RS09215 WP_002014817.1 1762732..1763166(+) (nucA/comI) [Bacillus mycoides strain 4BM1]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAGACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGACATTCGGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment