Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   V8V52_RS03310 Genome accession   NZ_CP150648
Coordinates   656800..658149 (+) Length   449 a.a.
NCBI ID   WP_088033457.1    Uniprot ID   -
Organism   Bacillus mycoides strain 4BM1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651800..663149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8V52_RS03290 (V8V52_03290) - 652717..653352 (-) 636 WP_088033387.1 YigZ family protein -
  V8V52_RS03295 (V8V52_03295) - 653743..654585 (+) 843 WP_002016143.1 DegV family protein -
  V8V52_RS03300 (V8V52_03300) - 654759..655073 (+) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  V8V52_RS03305 (V8V52_03305) - 655338..656669 (+) 1332 WP_088033385.1 C40 family peptidase -
  V8V52_RS03310 (V8V52_03310) comFA 656800..658149 (+) 1350 WP_088033457.1 ATP-dependent helicase ComFA Machinery gene
  V8V52_RS03315 (V8V52_03315) - 658149..658853 (+) 705 WP_088033383.1 ComF family protein -
  V8V52_RS03320 (V8V52_03320) cspC 658980..659177 (+) 198 WP_001990088.1 cold shock protein CspC -
  V8V52_RS03325 (V8V52_03325) raiA 659498..660040 (+) 543 WP_002016135.1 ribosome-associated translation inhibitor RaiA -
  V8V52_RS03330 (V8V52_03330) - 660419..661003 (+) 585 WP_088033381.1 hypothetical protein -
  V8V52_RS03335 (V8V52_03335) - 661035..661823 (+) 789 WP_088033379.1 hypothetical protein -
  V8V52_RS03340 (V8V52_03340) - 661810..662505 (+) 696 WP_088033378.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50871.22 Da        Isoelectric Point: 9.5337

>NTDB_id=970910 V8V52_RS03310 WP_088033457.1 656800..658149(+) (comFA) [Bacillus mycoides strain 4BM1]
MLAGRQLLLEELSSDLRNKLDHLKRNRDVVCVQGVIKKSSKYMCQRCGNIEQRLFVSLLCKRCNEVCTYCRKCITMGRVS
ECTVLVRGIAEISGESYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCSAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFQWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=970910 V8V52_RS03310 WP_088033457.1 656800..658149(+) (comFA) [Bacillus mycoides strain 4BM1]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCGGAATAAATTGGATCATCTGAAAAGGAATCG
AGACGTCGTTTGTGTACAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGTATCATTGCTATGTAAAAGGTGCAATGAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTAGTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCAGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTTTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTGTTAAAAATTTGCAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTTCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGACTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.34

98.218

0.494
  comFA Latilactobacillus sakei subsp. sakei 23K

41.206

88.641

0.365

Multiple sequence alignment