Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V8V52_RS01235 Genome accession   NZ_CP150648
Coordinates   224984..226360 (-) Length   458 a.a.
NCBI ID   WP_002009817.1    Uniprot ID   -
Organism   Bacillus mycoides strain 4BM1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 219984..231360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8V52_RS01215 (V8V52_01215) ispF 221356..221832 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  V8V52_RS01220 (V8V52_01220) ispD 221946..222626 (-) 681 WP_002009811.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  V8V52_RS01225 (V8V52_01225) - 222643..223746 (-) 1104 WP_002063406.1 PIN/TRAM domain-containing protein -
  V8V52_RS01230 (V8V52_01230) disA 223907..224980 (-) 1074 WP_000392162.1 DNA integrity scanning diadenylate cyclase DisA -
  V8V52_RS01235 (V8V52_01235) radA 224984..226360 (-) 1377 WP_002009817.1 DNA repair protein RadA Machinery gene
  V8V52_RS01240 (V8V52_01240) clpC 226455..228890 (-) 2436 WP_002009819.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  V8V52_RS01245 (V8V52_01245) - 228913..229977 (-) 1065 WP_002169332.1 protein arginine kinase -
  V8V52_RS01250 (V8V52_01250) - 229982..230530 (-) 549 WP_002009823.1 UvrB/UvrC motif-containing protein -
  V8V52_RS01255 (V8V52_01255) ctsR 230700..231161 (-) 462 WP_002029353.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49828.52 Da        Isoelectric Point: 7.4250

>NTDB_id=970899 V8V52_RS01235 WP_002009817.1 224984..226360(-) (radA) [Bacillus mycoides strain 4BM1]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVTQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPADAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=970899 V8V52_RS01235 WP_002009817.1 224984..226360(-) (radA) [Bacillus mycoides strain 4BM1]
ATGGCTAAAAAGAAAACAAAATTTACATGTCAAGAATGTGGTTATCAGTCGCCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TCAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGAGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCCATTCAGACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGGAGTGTAACGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGTGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGTCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCATATTTAAAAGTTGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TCGCCTTAAGTATTGCTTCAAGCTTCAGAGATAAAGCTACTGCACCAGCCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCGAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment