Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACLKZ2_RS03665 Genome accession   NZ_CP178225
Coordinates   754198..754794 (+) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain PA492     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 749198..759794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKZ2_RS03655 (ACLKZ2_03655) - 750478..752949 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  ACLKZ2_RS03660 (ACLKZ2_03660) pilM 753134..754198 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACLKZ2_RS03665 (ACLKZ2_03665) pilN 754198..754794 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACLKZ2_RS03670 (ACLKZ2_03670) pilO 754791..755414 (+) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLKZ2_RS03675 (ACLKZ2_03675) comL/pilP 755411..755935 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACLKZ2_RS03680 (ACLKZ2_03680) pilQ 755989..758128 (+) 2140 Protein_727 type 4a pilus secretin PilQ -
  ACLKZ2_RS03685 (ACLKZ2_03685) aroK 758140..758658 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=970894 ACLKZ2_RS03665 WP_003095839.1 754198..754794(+) (pilN) [Pseudomonas aeruginosa strain PA492]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=970894 ACLKZ2_RS03665 WP_003095839.1 754198..754794(+) (pilN) [Pseudomonas aeruginosa strain PA492]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGATTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369