Detailed information NTG0970862

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: ACLII2_RS20520
• Genome accession: NZ_CP178213
• Coordinates: 3911876..3912997 (-)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis subsp. subtilis strain BM107
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3906876..3917997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLII2_RS20500	znuC	3907385..3908080 (-)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-
ACLII2_RS20505	znuA	3908136..3909095 (-)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
ACLII2_RS20510	ycdG	3909283..3910968 (-)	1686	WP_003234736.1	glycoside hydrolase family 13 protein	-
ACLII2_RS20515	ycdF	3910993..3911769 (-)	777	WP_003246415.1	SDR family oxidoreductase	-
ACLII2_RS20520	rapF	3911876..3912997 (-)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
ACLII2_RS20525	cwlK	3913120..3913623 (+)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
ACLII2_RS20530	ycdC	3913631..3914992 (-)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
ACLII2_RS20535	ycdB	3915179..3916597 (-)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
ACLII2_RS20540	-	3916771..3916902 (+)	132	WP_003246334.1	hypothetical protein	-
ACLII2_RS20545	ycdA	3916925..3917989 (+)	1065	WP_003234750.1	DUF4352 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=970862 ACLII2_RS20520 WP_003246478.1 3911876..3912997(-) (rapF) [Bacillus subtilis subsp. subtilis strain BM107]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=970862 ACLII2_RS20520 WP_003246478.1 3911876..3912997(-) (rapF) [Bacillus subtilis subsp. subtilis strain BM107]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405