Detailed information NTG0970850

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: ACLII2_RS19320
• Genome accession: NZ_CP178213
• Coordinates: 3668948..3670123 (-)
• Length: 391 a.a.
• NCBI ID: WP_009966637.1
• Uniprot ID: P96649
• Organism: Bacillus subtilis subsp. subtilis strain BM107
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3663948..3675123

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLII2_RS19290	yddQ	3664271..3664813 (-)	543	WP_003234256.1	cysteine hydrolase family protein	-
ACLII2_RS19295	lrpB	3664928..3665377 (+)	450	WP_003246612.1	transcriptional regulator LrpB	-
ACLII2_RS19300	lrpA	3665500..3665910 (-)	411	WP_003246601.1	transcriptional regulator LrpA	-
ACLII2_RS19305	yddN	3666170..3667189 (+)	1020	WP_003234259.1	LLM class flavin-dependent oxidoreductase	-
ACLII2_RS19310	yddM	3667778..3668719 (-)	942	WP_010886416.1	helicase	-
ACLII2_RS19315	phrI	3668872..3668991 (-)	120	WP_010886415.1	phosphatase RapI inhibitor PhrI	-
ACLII2_RS19320	rapF	3668948..3670123 (-)	1176	WP_009966637.1	response regulator aspartate phosphatase RapI	Regulator
ACLII2_RS19325	spbK	3670463..3671263 (+)	801	WP_009966636.1	toll/interleukin-1 receptor domain-containing protein	-
ACLII2_RS19330	conJ	3671454..3671834 (-)	381	WP_009969670.1	DUF4467 domain-containing protein	-
ACLII2_RS19335	-	3671897..3672403 (-)	507	WP_009966634.1	hypothetical protein	-
ACLII2_RS19340	cwlT	3672418..3673407 (-)	990	WP_009966633.1	bifunctional lytic transglycosylase/C40 family peptidase	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 46566.15 Da
• Isoelectric Point: 4.9688

>NTDB_id=970850 ACLII2_RS19320 WP_009966637.1 3668948..3670123(-) (rapF) [Bacillus subtilis subsp. subtilis strain BM107]
MRGVFLDKDKIPYDLVTKKLNEWYTSIKNDQVEQAEIIKTEVEKELLNMEENQDALLYYQLLEFRHEIMLSYMKSKEIED
LNNAYETIKEIEKQGQLTGMLEYYFYFFKGMYEFRRKELISAISAYRIAESKLSEVEDEIEKAEFFFKVSYVYYYMKQTY
FSMNYANRALKIFREYEEYAVQTVRCQFIVAGNLIDSLEYERALEQFLKSLEISKESNIEHLIAMSHMNIGICYDELKEY
KKASQHLILALEIFEKSKHSFLTKTLFTLTYVEAKQQNYNVALIYFRKGRFIADKSDDKEYSAKFKILEGLFFSDGETQL
IKNAFSYLASRKMFADVENFSIEVADYFHEQGNLMLSNEYYRMSIEARRKIKKGEIIDENQPDSIGSSDFK

Nucleotide

• Length: 1176 bp

>NTDB_id=970850 ACLII2_RS19320 WP_009966637.1 3668948..3670123(-) (rapF) [Bacillus subtilis subsp. subtilis strain BM107]
TTGCGGGGTGTTTTCTTAGATAAAGATAAAATTCCGTACGACTTAGTCACGAAAAAGTTAAATGAATGGTATACATCAAT
AAAAAATGATCAAGTTGAGCAAGCCGAGATTATAAAAACAGAAGTAGAGAAAGAATTGTTAAACATGGAAGAAAATCAAG
ATGCCCTGTTATATTATCAACTATTAGAATTTAGACATGAGATAATGCTGAGTTATATGAAATCTAAGGAAATAGAAGAT
CTCAATAATGCTTATGAGACTATAAAAGAAATTGAGAAGCAAGGGCAATTAACTGGCATGTTGGAATACTATTTTTACTT
TTTTAAGGGTATGTACGAGTTTAGGCGTAAAGAATTAATTTCAGCGATAAGTGCTTATCGAATAGCTGAATCAAAGTTGT
CAGAAGTTGAGGATGAAATAGAGAAAGCAGAGTTTTTTTTCAAAGTGTCCTATGTATATTATTATATGAAACAAACATAC
TTCTCCATGAATTATGCAAATCGTGCACTCAAAATATTTAGAGAGTATGAAGAATATGCTGTCCAGACTGTGCGTTGTCA
ATTTATTGTAGCAGGAAACTTGATCGATTCATTGGAATATGAAAGAGCCTTGGAACAATTTTTGAAGTCTTTGGAAATTT
CCAAGGAAAGTAACATAGAGCATTTAATTGCAATGTCACATATGAATATTGGGATTTGTTATGATGAATTGAAAGAATAT
AAGAAGGCTTCACAACATTTAATTTTAGCGTTAGAAATTTTTGAAAAATCAAAACATAGTTTCTTAACAAAGACTTTATT
CACTCTAACCTATGTAGAAGCAAAACAACAAAATTATAATGTTGCTTTGATATACTTTAGGAAAGGGCGATTTATTGCCG
ATAAAAGTGATGATAAGGAATACTCAGCGAAATTCAAAATATTAGAGGGATTATTTTTTTCTGATGGTGAGACTCAATTA
ATAAAGAATGCATTTTCATATCTGGCTTCGAGAAAAATGTTTGCTGATGTTGAAAATTTTTCGATTGAAGTCGCTGATTA
TTTTCATGAACAAGGAAATTTAATGCTCTCTAATGAATATTATCGTATGAGTATTGAAGCAAGACGAAAAATTAAAAAAG
GGGAGATTATTGATGAAAATCAGCCGGATTCTATTGGCAGCAGTGATTTTAAGTAG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (10-70)

• Tetratricopeptide repeat (TPR_12) (199-256)

3D structure

• PDB: 4I1A

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	38.525	93.606	0.361
rapC	Bacillus subtilis subsp. subtilis str. 168	38.108	94.629	0.361