Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   VT188_RS23450 Genome accession   NZ_CP177358
Coordinates   5001460..5002953 (+) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain CWC0815     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4996460..5007953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT188_RS23420 (VT188_023420) - 4996672..4997481 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  VT188_RS23425 (VT188_023425) kdsD 4997895..4998875 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  VT188_RS23430 (VT188_023430) - 4998875..4999414 (+) 540 WP_003098849.1 KdsC family phosphatase -
  VT188_RS23435 (VT188_023435) lptC 4999423..4999995 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  VT188_RS23440 (VT188_023440) lptA 4999982..5000509 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  VT188_RS23445 (VT188_023445) lptB 5000509..5001234 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  VT188_RS23450 (VT188_023450) rpoN/rpoN1 5001460..5002953 (+) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  VT188_RS23455 (VT188_023455) hpf 5003031..5003339 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  VT188_RS23460 (VT188_023460) ptsN 5003353..5003817 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  VT188_RS23465 (VT188_023465) rapZ 5003819..5004679 (+) 861 WP_003094361.1 RNase adapter RapZ -
  VT188_RS23470 (VT188_023470) - 5004709..5004981 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  VT188_RS23475 (VT188_023475) - 5005078..5006010 (-) 933 WP_019485801.1 ZIP family metal transporter -
  VT188_RS23480 (VT188_023480) - 5006029..5006640 (-) 612 WP_003098862.1 superoxide dismutase -
  VT188_RS23485 (VT188_023485) - 5006653..5007102 (-) 450 WP_009875897.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=970511 VT188_RS23450 WP_003094357.1 5001460..5002953(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CWC0815]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=970511 VT188_RS23450 WP_003094357.1 5001460..5002953(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CWC0815]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGCT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477

Multiple sequence alignment