Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   ACLFK2_RS02250 Genome accession   NZ_CP177307
Coordinates   480628..480993 (+) Length   121 a.a.
NCBI ID   WP_003084587.1    Uniprot ID   A0A0H2ZKW2
Organism   Pseudomonas aeruginosa strain Pa021     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 475628..485993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLFK2_RS02220 (ACLFK2_02220) pyrR 476250..476762 (-) 513 WP_003084574.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACLFK2_RS02225 (ACLFK2_02225) ruvX 476874..477308 (-) 435 WP_003084575.1 Holliday junction resolvase RuvX -
  ACLFK2_RS02230 (ACLFK2_02230) - 477308..477877 (-) 570 WP_003100947.1 YqgE/AlgH family protein -
  ACLFK2_RS02235 (ACLFK2_02235) - 477926..478819 (-) 894 WP_003162015.1 energy transducer TonB -
  ACLFK2_RS02240 (ACLFK2_02240) gshB 478966..479919 (-) 954 WP_003162014.1 glutathione synthase -
  ACLFK2_RS02245 (ACLFK2_02245) pilG 480174..480581 (+) 408 WP_003084583.1 twitching motility response regulator PilG Regulator
  ACLFK2_RS02250 (ACLFK2_02250) pilH 480628..480993 (+) 366 WP_003084587.1 twitching motility response regulator PilH Machinery gene
  ACLFK2_RS02255 (ACLFK2_02255) - 481044..481580 (+) 537 WP_003084590.1 chemotaxis protein CheW -
  ACLFK2_RS02260 (ACLFK2_02260) pilJ 481665..483713 (+) 2049 WP_003084592.1 chemotaxis chemoreceptor PilJ -
  ACLFK2_RS02265 (ACLFK2_02265) pilK 483774..484649 (+) 876 WP_003084594.1 type 4 fimbrial methyltransferase PilK -

Sequence

Protein


Download         Length: 121 a.a.        Molecular weight: 13273.30 Da        Isoelectric Point: 5.1961

>NTDB_id=970355 ACLFK2_RS02250 WP_003084587.1 480628..480993(+) (pilH) [Pseudomonas aeruginosa strain Pa021]
MARILIVDDSPTEMYKLTAMLEKHGHQVLKAENGGDGVALARQEKPDVVLMDIVMPGLNGFQATRQLTKDAETSAIPVII
VTTKDQETDKVWGKRQGARDYLTKPVDEETLLKTINAVLAG

Nucleotide


Download         Length: 366 bp        

>NTDB_id=970355 ACLFK2_RS02250 WP_003084587.1 480628..480993(+) (pilH) [Pseudomonas aeruginosa strain Pa021]
ATGGCTCGTATTTTGATTGTTGATGACTCTCCGACCGAGATGTACAAGCTGACCGCCATGCTGGAAAAGCATGGTCACCA
GGTACTCAAGGCCGAGAACGGCGGCGACGGCGTCGCCCTGGCCCGCCAGGAAAAGCCCGACGTGGTCCTGATGGACATCG
TCATGCCCGGCCTCAACGGCTTCCAGGCGACCCGTCAATTGACCAAGGACGCCGAGACCAGCGCCATCCCGGTGATCATC
GTCACCACCAAGGACCAGGAGACCGACAAGGTCTGGGGCAAGCGCCAGGGCGCTCGCGACTACCTGACCAAGCCGGTGGA
CGAAGAGACCCTGCTGAAAACCATCAATGCGGTGCTGGCGGGCTGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZKW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

36.975

98.347

0.364