Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI25_RS23455 Genome accession   NZ_CP150307
Coordinates   5244186..5244623 (+) Length   145 a.a.
NCBI ID   WP_076302541.1    Uniprot ID   -
Organism   Paenibacillus sp. FSL E2-0177     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5239186..5249623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI25_RS23420 (MHI25_23420) - 5239274..5239960 (-) 687 WP_036683338.1 M15 family metallopeptidase -
  MHI25_RS23425 (MHI25_23425) - 5240078..5240242 (+) 165 WP_169744799.1 hypothetical protein -
  MHI25_RS23430 (MHI25_23430) - 5240239..5240574 (-) 336 WP_076128863.1 YolD-like family protein -
  MHI25_RS23435 (MHI25_23435) - 5240779..5241339 (-) 561 WP_340394465.1 hypothetical protein -
  MHI25_RS23440 (MHI25_23440) - 5241457..5241945 (+) 489 WP_076128858.1 PH domain-containing protein -
  MHI25_RS23445 (MHI25_23445) - 5242019..5243494 (-) 1476 WP_076185235.1 glycosyl hydrolase -
  MHI25_RS23450 (MHI25_23450) - 5243669..5244085 (-) 417 WP_038572821.1 cytidine deaminase -
  MHI25_RS23455 (MHI25_23455) nucA/comI 5244186..5244623 (+) 438 WP_076302541.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI25_RS23460 (MHI25_23460) - 5244705..5244854 (-) 150 WP_155288175.1 hypothetical protein -
  MHI25_RS23465 (MHI25_23465) - 5245004..5245180 (+) 177 WP_340394468.1 YjfB family protein -
  MHI25_RS23470 (MHI25_23470) - 5245278..5245895 (+) 618 WP_256716659.1 ImmA/IrrE family metallo-endopeptidase -
  MHI25_RS23475 (MHI25_23475) - 5245962..5246123 (+) 162 WP_155288176.1 hypothetical protein -
  MHI25_RS23480 (MHI25_23480) - 5246480..5246815 (+) 336 WP_076192025.1 DUF3139 domain-containing protein -
  MHI25_RS23485 (MHI25_23485) - 5246912..5247049 (+) 138 Protein_4633 phosphomethylpyrimidine synthase ThiC -
  MHI25_RS23490 (MHI25_23490) - 5247380..5247874 (+) 495 WP_248549356.1 sigma-70 family RNA polymerase sigma factor -
  MHI25_RS23495 (MHI25_23495) - 5247871..5248860 (+) 990 WP_339281312.1 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16140.06 Da        Isoelectric Point: 4.5847

>NTDB_id=970224 MHI25_RS23455 WP_076302541.1 5244186..5244623(+) (nucA/comI) [Paenibacillus sp. FSL E2-0177]
MKVKKWISSLIIVALLALASYWFEQNGEPTDPSSPSDSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENR
KESLKGIPTKKGYDRDEWPMAMCKEGGIGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=970224 MHI25_RS23455 WP_076302541.1 5244186..5244623(+) (nucA/comI) [Paenibacillus sp. FSL E2-0177]
TTGAAAGTAAAAAAATGGATCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGG
AGAGCCTACAGACCCCTCGTCTCCATCCGATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCG
CCAAACATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGT
AAAGAGTCCTTAAAAGGGATCCCCACTAAAAAGGGGTATGACCGGGATGAATGGCCAATGGCTATGTGTAAGGAAGGCGG
CATTGGTGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATT
ATGCAGACGGAACGCGGGTAGAATTTATGTTTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.903

71.034

0.497

Multiple sequence alignment