Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST25_RS23250 Genome accession   NZ_CP150306
Coordinates   5279835..5280272 (+) Length   145 a.a.
NCBI ID   WP_076128851.1    Uniprot ID   A0AAD0P503
Organism   Paenibacillus sp. FSL E2-0190     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5274835..5285272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST25_RS23215 (NST25_23215) - 5274912..5275598 (-) 687 WP_076220018.1 M15 family metallopeptidase -
  NST25_RS23220 (NST25_23220) - 5275716..5275880 (+) 165 WP_169744799.1 hypothetical protein -
  NST25_RS23225 (NST25_23225) - 5275877..5276212 (-) 336 WP_340392581.1 YolD-like family protein -
  NST25_RS23230 (NST25_23230) - 5276428..5276988 (-) 561 WP_076128860.1 hypothetical protein -
  NST25_RS23235 (NST25_23235) - 5277106..5277594 (+) 489 WP_076128858.1 PH domain-containing protein -
  NST25_RS23240 (NST25_23240) - 5277668..5279143 (-) 1476 WP_076279279.1 glycosyl hydrolase -
  NST25_RS23245 (NST25_23245) - 5279318..5279734 (-) 417 WP_038572821.1 cytidine deaminase -
  NST25_RS23250 (NST25_23250) nucA/comI 5279835..5280272 (+) 438 WP_076128851.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST25_RS23255 (NST25_23255) - 5280354..5280503 (-) 150 WP_155288175.1 hypothetical protein -
  NST25_RS23260 (NST25_23260) - 5280653..5280829 (+) 177 WP_076098563.1 YjfB family protein -
  NST25_RS23265 (NST25_23265) - 5280993..5281544 (+) 552 WP_036683410.1 ImmA/IrrE family metallo-endopeptidase -
  NST25_RS23270 (NST25_23270) - 5281611..5281772 (+) 162 WP_155288176.1 hypothetical protein -
  NST25_RS23275 (NST25_23275) - 5282133..5282468 (+) 336 WP_076279280.1 DUF3139 domain-containing protein -
  NST25_RS23280 (NST25_23280) - 5282583..5282702 (+) 120 Protein_4590 phosphomethylpyrimidine synthase ThiC -
  NST25_RS23285 (NST25_23285) - 5283033..5283527 (+) 495 WP_076136658.1 sigma-70 family RNA polymerase sigma factor -
  NST25_RS23290 (NST25_23290) - 5283524..5284513 (+) 990 WP_340392584.1 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16126.03 Da        Isoelectric Point: 4.5847

>NTDB_id=970187 NST25_RS23250 WP_076128851.1 5279835..5280272(+) (nucA/comI) [Paenibacillus sp. FSL E2-0190]
MKVKKWISSLIIVALLALASYWFEQNGEPTDPSSPSDSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENR
KESLKGIPTKKGYDRDEWPMAMCKEGGVGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=970187 NST25_RS23250 WP_076128851.1 5279835..5280272(+) (nucA/comI) [Paenibacillus sp. FSL E2-0190]
TTGAAAGTAAAAAAATGGATCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGG
AGAGCCTACAGACCCCTCGTCTCCATCCGATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCG
CCAAACATATTCAGGATGCCATTGCGAAAGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGT
AAAGAGTCCTTAAAAGGGATCCCCACTAAAAAGGGATATGACCGGGATGAATGGCCAATGGCTATGTGTAAGGAAGGCGG
CGTTGGTGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATT
ATGCAGACGGAACCCGGGTAGAATTTATGTTTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.903

71.034

0.497

Multiple sequence alignment