Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI51_RS23120 Genome accession   NZ_CP150298
Coordinates   5187286..5187717 (+) Length   143 a.a.
NCBI ID   WP_076220023.1    Uniprot ID   -
Organism   Paenibacillus sp. FSL H8-0260     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5182286..5192717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI51_RS23085 (MHI51_23015) - 5182358..5183044 (-) 687 WP_405173295.1 M15 family metallopeptidase -
  MHI51_RS23090 (MHI51_23020) - 5183162..5183326 (+) 165 WP_169744799.1 hypothetical protein -
  MHI51_RS23095 (MHI51_23025) - 5183323..5183658 (-) 336 WP_076128863.1 YolD-like family protein -
  MHI51_RS23100 (MHI51_23030) - 5183873..5184433 (-) 561 WP_076128860.1 hypothetical protein -
  MHI51_RS23105 (MHI51_23035) - 5184551..5185039 (+) 489 WP_076128858.1 PH domain-containing protein -
  MHI51_RS23110 (MHI51_23040) - 5185113..5186588 (-) 1476 WP_076220019.1 glycosyl hydrolase -
  MHI51_RS23115 (MHI51_23045) - 5186763..5187179 (-) 417 WP_038572821.1 cytidine deaminase -
  MHI51_RS23120 (MHI51_23050) nucA/comI 5187286..5187717 (+) 432 WP_076220023.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI51_RS23125 (MHI51_23055) - 5187799..5187948 (-) 150 WP_155288175.1 hypothetical protein -
  MHI51_RS23130 (MHI51_23060) - 5188098..5188274 (+) 177 WP_076098563.1 YjfB family protein -
  MHI51_RS23135 (MHI51_23065) - 5188438..5188989 (+) 552 WP_076220024.1 ImmA/IrrE family metallo-endopeptidase -
  MHI51_RS23140 (MHI51_23070) - 5189056..5189217 (+) 162 WP_155288176.1 hypothetical protein -
  MHI51_RS23145 (MHI51_23075) - 5189583..5189918 (+) 336 WP_076220020.1 DUF3139 domain-containing protein -
  MHI51_RS23150 (MHI51_23080) - 5190032..5190124 (+) 93 Protein_4565 phosphomethylpyrimidine synthase ThiC -
  MHI51_RS23155 (MHI51_23085) - 5190483..5190977 (+) 495 WP_076220021.1 RNA polymerase sigma factor -
  MHI51_RS23160 (MHI51_23090) - 5190974..5191963 (+) 990 WP_342410053.1 hypothetical protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15886.67 Da        Isoelectric Point: 4.4812

>NTDB_id=969906 MHI51_RS23120 WP_076220023.1 5187286..5187717(+) (nucA/comI) [Paenibacillus sp. FSL H8-0260]
MKKWTSSLIIVALLALASYWFEQNGEPTDPSSPSDSSVVQLTFPSDRYPETAKHIQDAIAKGESATCTINREQAEENRKE
SLKGIPTKKGYDRDEWPMAMCKEGGVGADIEYITPSDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=969906 MHI51_RS23120 WP_076220023.1 5187286..5187717(+) (nucA/comI) [Paenibacillus sp. FSL H8-0260]
GTGAAAAAATGGACCTCCAGTCTCATCATTGTTGCATTACTTGCTTTGGCTAGTTACTGGTTTGAACAAAACGGAGAGCC
TACAGACCCCTCGTCTCCATCCGATTCGAGTGTTGTTCAGCTAACCTTCCCATCAGACCGCTATCCTGAGACCGCCAAAC
ATATTCAGGATGCCATTGCGAAGGGTGAATCCGCCACATGTACGATTAACCGAGAGCAGGCTGAAGAGAACCGTAAAGAG
TCCTTAAAAGGGATTCCCACTAAAAAGGGGTATGACCGGGATGAATGGCCAATGGCTATGTGTAAGGAAGGCGGCGTTGG
TGCCGACATTGAATACATAACGCCAAGCGATAACCGCGGGGCAGGAAGCTGGGTTGGCAATCAGCTGGAGGATTATGCAG
ACGGAACCCGGGTAGAATTTATGTTTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.903

72.028

0.503

Multiple sequence alignment