Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI27_RS23285 Genome accession   NZ_CP150297
Coordinates   5230360..5230791 (+) Length   143 a.a.
NCBI ID   WP_042194351.1    Uniprot ID   A0A089IM67
Organism   Paenibacillus sp. FSL H8-0261     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5225360..5235791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI27_RS23255 (MHI27_23205) - 5226060..5226224 (+) 165 WP_179085776.1 hypothetical protein -
  MHI27_RS23260 (MHI27_23210) - 5226221..5226556 (-) 336 WP_042130171.1 YolD-like family protein -
  MHI27_RS23265 (MHI27_23215) - 5226939..5227499 (-) 561 WP_149646645.1 hypothetical protein -
  MHI27_RS23270 (MHI27_23220) - 5227617..5228105 (+) 489 WP_339797888.1 PH domain-containing protein -
  MHI27_RS23275 (MHI27_23225) - 5228187..5229662 (-) 1476 WP_405174812.1 glycosyl hydrolase -
  MHI27_RS23280 (MHI27_23230) - 5229836..5230252 (-) 417 WP_042194350.1 cytidine deaminase -
  MHI27_RS23285 (MHI27_23235) nucA/comI 5230360..5230791 (+) 432 WP_042194351.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI27_RS23290 (MHI27_23240) - 5230870..5231016 (-) 147 WP_156113170.1 hypothetical protein -
  MHI27_RS23295 (MHI27_23245) - 5231166..5231342 (+) 177 WP_076098563.1 YjfB family protein -
  MHI27_RS23300 (MHI27_23250) - 5231483..5232025 (+) 543 WP_405174813.1 ImmA/IrrE family metallo-endopeptidase -
  MHI27_RS23305 (MHI27_23255) - 5232072..5232263 (+) 192 WP_341114452.1 hypothetical protein -
  MHI27_RS23310 (MHI27_23260) - 5232388..5232561 (-) 174 WP_341146105.1 hypothetical protein -
  MHI27_RS23315 (MHI27_23265) - 5232670..5233002 (+) 333 WP_405174814.1 DUF3139 domain-containing protein -
  MHI27_RS23320 (MHI27_23270) - 5233176..5233670 (+) 495 WP_042190786.1 RNA polymerase sigma factor -
  MHI27_RS23325 (MHI27_23275) - 5233667..5234656 (+) 990 WP_405174815.1 hypothetical protein -
  MHI27_RS23330 (MHI27_23280) - 5234892..5235020 (-) 129 WP_405174816.1 YjcZ family sporulation protein -
  MHI27_RS23335 (MHI27_23285) - 5235330..5235434 (-) 105 WP_231573505.1 YjcZ family sporulation protein -
  MHI27_RS23340 (MHI27_23290) - 5235465..5235614 (-) 150 WP_197071333.1 dihydroorotate dehydrogenase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15851.71 Da        Isoelectric Point: 4.4729

>NTDB_id=969869 MHI27_RS23285 WP_042194351.1 5230360..5230791(+) (nucA/comI) [Paenibacillus sp. FSL H8-0261]
MQKLLTSLIIVVLLALGGYWYEQNGDPAAPSSTSDSEVVQLIFPSDRYPETAKHIQDAIAKGESATCTINREQAEDNRKE
SLKGIPTKKGYDRDEWPMAMCNEGGKGADIEYITPKDNRGAGSWVGNQLEDYADGTRVEFMFK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=969869 MHI27_RS23285 WP_042194351.1 5230360..5230791(+) (nucA/comI) [Paenibacillus sp. FSL H8-0261]
ATACAGAAATTACTCACCAGTCTCATCATTGTTGTGTTACTTGCTTTGGGCGGTTACTGGTATGAACAGAACGGAGATCC
GGCAGCCCCCTCGTCCACATCCGATTCAGAAGTTGTTCAGCTGATCTTCCCATCAGATCGTTATCCTGAGACCGCTAAGC
ACATTCAGGACGCAATTGCTAAAGGGGAGTCAGCGACGTGCACGATTAACCGTGAGCAGGCTGAAGACAACCGCAAAGAA
TCCTTAAAAGGGATCCCCACCAAAAAAGGCTACGACCGCGATGAATGGCCCATGGCCATGTGTAATGAAGGCGGCAAAGG
GGCCGACATTGAATACATAACCCCAAAAGATAACCGCGGTGCAGGAAGCTGGGTTGGCAATCAGTTAGAAGATTATGCGG
ATGGAACCCGCGTAGAATTTATGTTCAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A089IM67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.961

72.028

0.49

Multiple sequence alignment