Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHB44_RS22555 Genome accession   NZ_CP150292
Coordinates   4672263..4673639 (-) Length   458 a.a.
NCBI ID   WP_053997206.1    Uniprot ID   -
Organism   Lysinibacillus sp. FSL H8-0500     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4667263..4678639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB44_RS22535 (MHB44_22535) ispF 4667845..4668321 (-) 477 WP_341300958.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MHB44_RS22540 (MHB44_22540) - 4668499..4670133 (+) 1635 WP_341300959.1 SWIM zinc finger family protein -
  MHB44_RS22545 (MHB44_22545) ispD 4670190..4670882 (-) 693 WP_341300960.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHB44_RS22550 (MHB44_22550) - 4670894..4671979 (-) 1086 WP_341300961.1 PIN/TRAM domain-containing protein -
  MHB44_RS22555 (MHB44_22555) radA 4672263..4673639 (-) 1377 WP_053997206.1 DNA repair protein RadA Machinery gene
  MHB44_RS22560 (MHB44_22560) - 4673729..4676173 (-) 2445 WP_341300962.1 ATP-dependent Clp protease ATP-binding subunit -
  MHB44_RS22565 (MHB44_22565) - 4676163..4677275 (-) 1113 WP_341300963.1 protein arginine kinase -
  MHB44_RS22570 (MHB44_22570) - 4677272..4677814 (-) 543 WP_341300964.1 UvrB/UvrC motif-containing protein -
  MHB44_RS22575 (MHB44_22575) - 4677825..4678298 (-) 474 WP_341300965.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50193.09 Da        Isoelectric Point: 7.6802

>NTDB_id=969748 MHB44_RS22555 WP_053997206.1 4672263..4673639(-) (radA) [Lysinibacillus sp. FSL H8-0500]
MAKKKTKFICSGCGYESAKWMGRCPGCGEWNKMVEEVEVVAKGPRGAFQHSATVTQKALPIIQVQATEESRVATEMSELN
RVLGGGIVAGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVVSQELYIYSETNLEFLHQ
TIDEVQPKFVIVDSIQTIFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVQATDCFIGEVGLTG
EVRRVSRIEQRVIEAAKLGFKRAFIPASNIGGWDFPQGIEIVGVETIKDALKLCFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=969748 MHB44_RS22555 WP_053997206.1 4672263..4673639(-) (radA) [Lysinibacillus sp. FSL H8-0500]
TTGGCAAAGAAAAAAACGAAATTTATCTGTTCGGGCTGTGGCTATGAATCTGCAAAATGGATGGGAAGATGTCCAGGCTG
TGGAGAATGGAATAAGATGGTGGAGGAAGTTGAGGTTGTGGCAAAAGGACCTCGTGGAGCATTCCAGCATTCCGCAACAG
TGACCCAAAAGGCACTGCCCATTATTCAGGTCCAGGCCACGGAAGAGTCTCGTGTAGCAACGGAAATGAGCGAACTGAAT
CGTGTGCTAGGTGGAGGAATTGTGGCTGGCTCGTTAGTGTTAATTGGGGGTGATCCTGGGATTGGGAAATCAACGCTTTT
ATTACAAGTTTCAGCATTGCTCTCAAATAAAGGGCATCGTGTGCTTTATATTTCTGGAGAGGAATCAATTCGTCAGACAA
AATTACGAGCAGAACGTTTAGGCGTAGTTTCTCAAGAGCTATATATCTATTCTGAAACGAATCTTGAATTTTTACATCAA
ACGATTGATGAGGTGCAGCCGAAATTTGTCATTGTTGATTCTATACAGACGATCTTTCATCCTGAAGTTACAAGCGCACC
AGGGAGTGTTTCACAGGTACGAGAATGTACAGCTGAGCTAATGCGTATAGCCAAAACAAAGGGGATTGCTATCTTTTTAG
TGGGGCATGTTACAAAAGAAGGACAAATTGCTGGACCACGTATTTTAGAGCATATGGTGGATACGGTATTGTATTTTGAA
GGAGAAAGACATCATAATCATCGTATTTTACGGAGCCAAAAAAATCGCTTTGGCTCTACCAATGAAATTGCGATATTTGA
GATGCTTCAGGGAGGTTTAAAGGAAGTGCTAAATCCTTCTGAGCTATTTTTACAGGAACGCTCACAGGGAGCTGCGGGGT
CTACGATCGTGGCGTCAATGGAGGGGACAAGACCTATTCTTGTTGAAATTCAATCTTTAGTAACGCCTACAAGCTTTAAT
TATCCCAAACGTATGGCAACGGGTGTTGATCAAAATCGGGTGCAGTTGCTGATGGCGGTGCTTGAAAAGCGTATGGGGCT
TATGCTGCAAGCGCAAGATGCATATATTAAAGTTGCTGGTGGTGTGAAATTGGATGAGCCCGCCATTGATTTAGCTGTGT
TGACAAGTATTGTCTCGAGCTTTAAGGATCAAGCGGTACAAGCAACAGATTGTTTTATTGGAGAGGTCGGATTGACAGGA
GAGGTACGTCGAGTCTCACGTATTGAGCAGCGTGTTATTGAAGCCGCGAAACTTGGATTTAAAAGGGCTTTTATTCCAGC
ATCTAATATCGGTGGATGGGATTTCCCCCAAGGTATTGAAATTGTAGGAGTAGAAACGATCAAGGATGCCTTAAAACTCT
GCTTTAGAGAGCTATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-221)

Subunit ChlI of Mg-chelatase (ChlI)

(346-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.11

99.345

0.697
  radA Streptococcus pneumoniae Rx1

62.281

99.563

0.62
  radA Streptococcus pneumoniae D39

62.281

99.563

0.62
  radA Streptococcus pneumoniae R6

62.281

99.563

0.62
  radA Streptococcus pneumoniae TIGR4

62.281

99.563

0.62
  radA Streptococcus mitis NCTC 12261

62.281

99.563

0.62
  radA Streptococcus mitis SK321

62.281

99.563

0.62

Multiple sequence alignment