Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AACH74_RS01960 Genome accession   NZ_AP029000
Coordinates   421109..421786 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain GU2018_CL13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416109..426786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH74_RS01945 (GU2018CL13_03850) chiA 417467..420169 (+) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -
  AACH74_RS01950 (GU2018CL13_03860) bfd 420338..420532 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AACH74_RS01955 (GU2018CL13_03870) bfr 420604..421080 (+) 477 WP_000675504.1 bacterioferritin -
  AACH74_RS01960 (GU2018CL13_03880) pilD 421109..421786 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  AACH74_RS01965 (GU2018CL13_03890) gspM 421786..422247 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  AACH74_RS01970 (GU2018CL13_03900) gspL 422244..423407 (-) 1164 WP_001332436.1 type II secretion system protein GspL -
  AACH74_RS01975 (GU2018CL13_03910) gspK 423422..424405 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  AACH74_RS01980 (GU2018CL13_03920) gspJ 424398..424985 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  AACH74_RS01985 (GU2018CL13_03930) gspI 424978..425373 (-) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  AACH74_RS01990 (GU2018CL13_03940) gspH 425370..425879 (-) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  AACH74_RS01995 (GU2018CL13_03950) gspG 425887..426324 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=96971 AACH74_RS01960 WP_000178175.1 421109..421786(-) (pilD) [Escherichia coli strain GU2018_CL13]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=96971 AACH74_RS01960 WP_000178175.1 421109..421786(-) (pilD) [Escherichia coli strain GU2018_CL13]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387

Multiple sequence alignment