Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHB62_RS12735 Genome accession   NZ_CP150278
Coordinates   2480978..2481388 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. FSL K6-0255     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2475978..2486388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB62_RS12705 (MHB62_12705) - 2476090..2476230 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MHB62_RS12710 (MHB62_12710) - 2476536..2477267 (-) 732 WP_032722145.1 SGNH/GDSL hydrolase family protein -
  MHB62_RS12715 (MHB62_12715) cwlH 2477519..2478271 (-) 753 WP_032722147.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MHB62_RS12720 (MHB62_12720) - 2478458..2479084 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  MHB62_RS12725 (MHB62_12725) gnd 2479103..2479971 (-) 869 Protein_2456 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MHB62_RS12730 (MHB62_12730) - 2480223..2480945 (+) 723 WP_010886572.1 hypothetical protein -
  MHB62_RS12735 (MHB62_12735) nucA/comI 2480978..2481388 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MHB62_RS12740 (MHB62_12740) - 2481584..2481934 (+) 351 Protein_2459 sigma-70 family RNA polymerase sigma factor -
  MHB62_RS12745 (MHB62_12745) spoIVCA 2481962..2483422 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  MHB62_RS12750 (MHB62_12750) - 2483380..2483558 (-) 179 Protein_2461 hypothetical protein -
  MHB62_RS12755 (MHB62_12755) - 2483913..2484332 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  MHB62_RS12760 (MHB62_12760) acr3 2484344..2485384 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  MHB62_RS12765 (MHB62_12765) - 2485407..2485847 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  MHB62_RS12770 (MHB62_12770) arsR 2485906..2486223 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=969275 MHB62_RS12735 WP_009967785.1 2480978..2481388(-) (nucA/comI) [Bacillus sp. FSL K6-0255]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=969275 MHB62_RS12735 WP_009967785.1 2480978..2481388(-) (nucA/comI) [Bacillus sp. FSL K6-0255]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment