Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   MHH79_RS13255 Genome accession   NZ_CP150275
Coordinates   2544335..2544745 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. FSL K6-0993     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 2539335..2549745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH79_RS13225 (MHH79_13225) - 2539422..2539562 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MHH79_RS13230 (MHH79_13230) - 2539868..2540599 (-) 732 WP_047182903.1 SGNH/GDSL hydrolase family protein -
  MHH79_RS13235 (MHH79_13235) cwlH 2540851..2541603 (-) 753 WP_340034618.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MHH79_RS13240 (MHH79_13240) - 2541790..2542416 (+) 627 WP_340034620.1 TVP38/TMEM64 family protein -
  MHH79_RS13245 (MHH79_13245) gnd 2542435..2543328 (-) 894 WP_015384138.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MHH79_RS13250 (MHH79_13250) - 2543580..2544302 (+) 723 WP_015384139.1 hypothetical protein -
  MHH79_RS13255 (MHH79_13255) nucA/comI 2544335..2544745 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MHH79_RS13260 (MHH79_13260) sigK 2544941..2545669 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  MHH79_RS13265 (MHH79_13265) - 2545751..2545942 (-) 192 Protein_2562 recombinase family protein -
  MHH79_RS13270 (MHH79_13270) - 2545900..2546064 (-) 165 WP_015483452.1 hypothetical protein -
  MHH79_RS13275 (MHH79_13275) - 2546520..2546615 (-) 96 Protein_2564 phosphate-starvation-inducible protein PsiE -
  MHH79_RS13280 (MHH79_13280) yrkQ 2546660..2547958 (-) 1299 WP_063335458.1 two-component system sensor histidine kinase YrkQ -
  MHH79_RS13285 (MHH79_13285) yrkP 2547945..2548640 (-) 696 WP_340034622.1 two-component response regulator YrkP -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=969122 MHH79_RS13255 WP_009967785.1 2544335..2544745(-) (nucA/comI) [Bacillus sp. FSL K6-0993]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=969122 MHH79_RS13255 WP_009967785.1 2544335..2544745(-) (nucA/comI) [Bacillus sp. FSL K6-0993]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGTGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529


Multiple sequence alignment