Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACK8N4_RS28705 Genome accession   NZ_CP176811
Coordinates   6136216..6138435 (+) Length   739 a.a.
NCBI ID   WP_124132624.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain DG-1     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6131216..6143435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8N4_RS28685 (ACK8N4_28685) - 6132191..6133459 (-) 1269 WP_003103278.1 malic enzyme -
  ACK8N4_RS28690 (ACK8N4_28690) - 6133562..6135001 (-) 1440 WP_124132625.1 M48 family metalloprotease -
  ACK8N4_RS28695 (ACK8N4_28695) - 6134998..6135765 (-) 768 WP_025271418.1 thermonuclease family protein -
  ACK8N4_RS28700 (ACK8N4_28700) rpmE 6135825..6136040 (-) 216 WP_003095846.1 50S ribosomal protein L31 -
  ACK8N4_RS28705 (ACK8N4_28705) priA 6136216..6138435 (+) 2220 WP_124132624.1 primosomal protein N' Machinery gene
  ACK8N4_RS28710 (ACK8N4_28710) argS 6138684..6140447 (+) 1764 WP_003135774.1 arginine--tRNA ligase -
  ACK8N4_RS28715 (ACK8N4_28715) - 6140482..6141177 (+) 696 WP_039026604.1 SPOR domain-containing protein -
  ACK8N4_RS28720 (ACK8N4_28720) hslV 6141296..6141829 (+) 534 WP_003095852.1 ATP-dependent protease subunit HslV -
  ACK8N4_RS28725 (ACK8N4_28725) hslU 6141858..6143201 (+) 1344 WP_003095854.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82137.65 Da        Isoelectric Point: 8.9189

>NTDB_id=969110 ACK8N4_RS28705 WP_124132624.1 6136216..6138435(+) (priA) [Pseudomonas aeruginosa strain DG-1]
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLVPFGRRELVGVLIEMTDRSEVPEDKLKPALRVLDAKPPMPAH
LLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHATAQSSLDDPRLARAPRQRQALAILKQHPHGVSHELL
NQLQINKDSLDLLKEKGLVELEVRRHSTPPREGGWLAQAELPLNPEQRAAFEAVRASHGGFHCFLLAGVTGSGKTEVYLQ
LIRETLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRERLDAWLAARDGEADIVIGTRSALFTPLKRPGL
IIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGSATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKS
MPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNC
PQCGKLDLRPVGAGTERAEERLRILFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDSACSAAEQLLEQLGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=969110 ACK8N4_RS28705 WP_124132624.1 6136216..6138435(+) (priA) [Pseudomonas aeruginosa strain DG-1]
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGCGGCATTCC
GCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGGTGCCGTTCGGGCGCCGGGAGCTGGTTGGCGTTCTGATAGAGATGA
CGGATCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGACGCCAAGCCGCCGATGCCGGCGCAC
CTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTCGGCGACACCCTGTCCTGGGCGCTGCCCAACCT
GTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGCTTCTGGCATGCCACCGCACAATCCAGCCTCGACGATCCGC
GCCTGGCCCGCGCACCGCGCCAGCGCCAGGCCCTGGCGATTCTCAAGCAGCATCCGCACGGGGTGTCCCATGAGTTGCTG
AACCAGTTGCAGATAAACAAGGACAGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACAG
CACCCCGCCGCGCGAAGGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAGGCGG
TCCGCGCCAGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGCCGCAGGCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGGTCCGCAGACCCTGGC
GCGTTTCGAGCGCCGCTTCAACGCGCGCATCGCCCTGCTGCACTCGGCCCTGACCGACCGCGAGCGCCTCGACGCCTGGC
TCGCCGCCCGGGATGGCGAGGCCGATATCGTCATCGGCACCCGCTCGGCACTGTTCACTCCGCTGAAGCGGCCGGGACTG
ATCATCGTCGATGAGGAACACGACGCCTCCTATAAACAGCAGGACGGCCTGCGCTACCACGCCCGCGACCTGGCACTGGT
ACGCGCGCGCCTGGAGAACGTGCCGATCCTGCTCGGTTCGGCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCG
GCCGCTACGGCCTGCTGCGCCTGACCCAGCGCGCCGGTGGCGCGCACCCGCCGAAGTTCATCCGCCTCGACGTGAAGAGC
ATGCCGCTCGACGCCGGCCTTTCCCGTCCGCTGCAACAGGCTATCGGCGATACCCTGGCGGCCGGCCAGCAGGTTCTGGT
GTTCCTCAACCGCCGCGGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACG
CGCGGATGACCGTGCATCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAATGCGGCAAGCTCGACCTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATTCTCTTCCC
GAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTCTTCGCCACCATCAACA
GCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTCCCACGGGTCACCCTGGTGGCGATC
CTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAACGCATGGCCCAGCAGATCGTCCAGGTCGCCGG
CCGCGCAGGACGCGCCGAGGAGCCCGGACGGGTACTGATCCAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCA
CCGAGGACGGCTACTTCGCCTTTGCCGAACAGGCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTC
GCCCTGCTGCGCGCCGAAGCGCACAAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATT
GCTCGAGCAGCTGGGCGGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACC
GCGCGCAGCTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCTTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCACTCGATATCGACCCGATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Helicase conserved C-terminal domain (Helicase_C)

(522-592)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.613

99.188

0.472

Multiple sequence alignment