Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACK8N4_RS01990 Genome accession   NZ_CP176811
Coordinates   427137..428510 (+) Length   457 a.a.
NCBI ID   WP_411387741.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain DG-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422137..433510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8N4_RS01970 (ACK8N4_01970) - 422974..423540 (-) 567 WP_033974084.1 hypothetical protein -
  ACK8N4_RS01975 (ACK8N4_01975) rsmD 423543..424139 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACK8N4_RS01980 (ACK8N4_01980) - 424139..425626 (-) 1488 WP_033974085.1 M16 family metallopeptidase -
  ACK8N4_RS01985 (ACK8N4_01985) - 425619..427016 (-) 1398 WP_073649045.1 M16 family metallopeptidase -
  ACK8N4_RS01990 (ACK8N4_01990) pilA 427137..428510 (+) 1374 WP_411387741.1 signal recognition particle-docking protein FtsY Machinery gene
  ACK8N4_RS01995 (ACK8N4_01995) ftsE 428507..429178 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  ACK8N4_RS02000 (ACK8N4_02000) ftsX 429178..430185 (+) 1008 WP_033974094.1 permease-like cell division protein FtsX -
  ACK8N4_RS02005 (ACK8N4_02005) rpoH 430298..431152 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  ACK8N4_RS02010 (ACK8N4_02010) - 431217..431822 (+) 606 WP_033974096.1 hypothetical protein -
  ACK8N4_RS02015 (ACK8N4_02015) mtgA 431827..432558 (-) 732 WP_073650409.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACK8N4_RS02020 (ACK8N4_02020) - 432595..432972 (+) 378 WP_033974098.1 DUF423 domain-containing protein -
  ACK8N4_RS02025 (ACK8N4_02025) thiS 433075..433275 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 48560.06 Da        Isoelectric Point: 4.5525

>NTDB_id=969029 ACK8N4_RS01990 WP_411387741.1 427137..428510(+) (pilA) [Pseudomonas aeruginosa strain DG-1]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETSAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPPAP
VAEAPLAGDGPVSAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPEPVPVAPLAAAPAVSEPATRPGFFARLRQGLS
KTSASIGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVE
QPLQVDVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSAS
VIFDAVQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTG
LALTKLDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=969029 ACK8N4_RS01990 WP_411387741.1 427137..428510(+) (pilA) [Pseudomonas aeruginosa strain DG-1]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACGTCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGATGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGCCGGCGCCG
GTTGCCGAAGCGCCGCTCGCCGGTGACGGGCCAGTGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCA
ACCCGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTGCCCGTTG
CGCCGCTGGCCGCCGCTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCC
AAGACCAGCGCCAGCATCGGCGAAGGCATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGA
CATCGAGACGCGCCTGCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGG
CGCGCAAGGAGCTGGCCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAG
CAGCCGTTGCAGGTCGACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCAC
CATCGGCAAGCTGGCGAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCG
CCGTGGAGCAGTTGCAGGTCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCTTCG
GTGATCTTCGACGCGGTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATAC
CAAGGACAATCTGATGGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAAACGGCGCCCCATGAGGTCC
TGCTGGTGCTCGACGCCGGTACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGC
CTGGCCCTGACCAAACTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCG
TTATATCGGTGTCGGCGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGATGCCTTCGTCCAGGCACTCTTCGCAGAGC
GGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(167-232)

SRP54-type protein, GTPase domain (SRP54)

(252-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53.488

75.274

0.403

Multiple sequence alignment