Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACK28Q_RS43225 Genome accession   NZ_CP176762
Coordinates   9112822..9114435 (-) Length   537 a.a.
NCBI ID   WP_014490898.1    Uniprot ID   A0ABV2RJG5
Organism   Bradyrhizobium japonicum strain DSM 3014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9107822..9119435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK28Q_RS43210 (ACK28Q_43210) - 9109443..9111179 (-) 1737 WP_014490900.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -
  ACK28Q_RS43215 (ACK28Q_43215) ptsN 9111455..9111916 (-) 462 WP_008141251.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACK28Q_RS43220 (ACK28Q_43220) hpf 9112138..9112737 (-) 600 WP_014490899.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACK28Q_RS43225 (ACK28Q_43225) rpoN/rpoN1 9112822..9114435 (-) 1614 WP_014490898.1 RNA polymerase factor sigma-54 Machinery gene
  ACK28Q_RS43230 (ACK28Q_43230) lptB 9114526..9115536 (-) 1011 WP_014490897.1 LPS export ABC transporter ATP-binding protein -
  ACK28Q_RS43235 (ACK28Q_43235) - 9115783..9116499 (-) 717 WP_014490896.1 LptA/OstA family protein -
  ACK28Q_RS43240 (ACK28Q_43240) lptC 9116505..9117236 (-) 732 WP_028169711.1 LPS export ABC transporter periplasmic protein LptC -
  ACK28Q_RS43245 (ACK28Q_43245) - 9117382..9117996 (-) 615 WP_014490894.1 ribonuclease D -

Sequence

Protein


Download         Length: 537 a.a.        Molecular weight: 58988.08 Da        Isoelectric Point: 4.5479

>NTDB_id=968782 ACK28Q_RS43225 WP_014490898.1 9112822..9114435(-) (rpoN/rpoN1) [Bradyrhizobium japonicum strain DSM 3014]
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLTTFVEEELERNPLLERANDEAPAGEAPAETGQFSDSDGRHNDEPG
GGPGEAFEPGQEEWMSKDLGTRAEIEQTLDTGLDNVFSEEPAEAAARNAQDAAPTTYTEWGGGASGDEDYNLEAFVAAEI
TLGDHLAEQLSVAFTGPAQRMIGQYLIDLVDEAGYLPPDLGQAAERLGASQADVEDVLAVLQKFDPPGVCARNLSECLAI
QLRELDRYDPAMQALVEHLDLLAKRDIASLRKLCGVDDEDIADMIGEIRRLNPKPGMKFGSARLQTMVPDVYVRPGPDGG
WHVELNSDTLPRVLVNQTYYSELSKKIGKDGDKSYFTDALQNATWLVRALDQRARTILKVATEIVRQQDGFFTHGVAHLR
PLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIKQLIDSEAPAAILSDDTI
VERLRASGIDIARRTVAKYREAMRIPSSVQRRRDKQSALGNVLSTAMPDRSRNTEPA

Nucleotide


Download         Length: 1614 bp        

>NTDB_id=968782 ACK28Q_RS43225 WP_014490898.1 9112822..9114435(-) (rpoN/rpoN1) [Bradyrhizobium japonicum strain DSM 3014]
ATGGCGCTCACGCAGAGATTAGAGTTCCGGCAATCGCAGTCGCTGGTCATGACGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAATTGTCCAATCTCGATCTCACGACCTTCGTGGAAGAGGAACTCGAGCGGAACCCCCTGCTGGAGCGGGCCAACG
ACGAGGCCCCCGCGGGTGAAGCCCCGGCCGAGACCGGCCAGTTCAGCGATTCCGACGGGAGGCACAACGACGAGCCGGGC
GGCGGTCCGGGCGAGGCGTTCGAGCCGGGCCAGGAAGAATGGATGAGCAAGGATCTCGGCACCCGCGCCGAGATCGAGCA
GACCCTGGACACGGGCCTGGACAACGTCTTCTCCGAGGAACCGGCCGAGGCCGCCGCGCGCAACGCCCAGGATGCGGCGC
CGACCACCTATACCGAATGGGGCGGCGGCGCCTCCGGCGACGAGGATTACAATCTCGAAGCCTTCGTCGCCGCGGAGATC
ACGCTCGGTGACCATCTTGCCGAGCAGCTGTCGGTCGCCTTCACCGGCCCGGCGCAGCGCATGATCGGCCAGTACCTGAT
CGACCTCGTCGACGAGGCCGGCTATCTGCCGCCGGATCTCGGCCAGGCCGCCGAGCGCCTTGGCGCATCGCAAGCCGACG
TCGAGGACGTCCTTGCCGTGCTGCAAAAATTCGATCCGCCCGGCGTCTGCGCGCGCAATTTGAGCGAATGCCTGGCGATC
CAGCTCCGCGAGCTCGACCGCTACGACCCGGCGATGCAGGCCCTCGTCGAGCACCTCGATCTGCTGGCGAAGCGCGACAT
CGCTTCGTTGCGCAAGCTCTGCGGCGTCGACGACGAGGACATCGCCGACATGATCGGCGAGATCCGCCGCCTCAATCCCA
AGCCCGGCATGAAGTTCGGCTCGGCGCGGCTCCAGACCATGGTGCCCGACGTCTATGTCCGCCCGGGCCCGGATGGCGGC
TGGCATGTCGAGCTCAACAGCGACACCTTGCCGCGCGTGCTGGTCAACCAGACCTATTATTCCGAGCTGTCGAAGAAGAT
CGGCAAGGACGGCGACAAGTCCTATTTCACCGACGCGCTCCAGAACGCGACCTGGCTGGTGCGCGCGCTCGATCAGCGGG
CCCGCACCATCCTGAAAGTTGCAACGGAAATCGTGCGCCAGCAGGACGGCTTCTTCACCCACGGCGTGGCGCATCTGCGG
CCGCTGAATCTGAAGGCCGTGGCCGACGCCATCCAGATGCATGAATCCACGGTGTCGCGCGTCACCGCCAACAAGTACAT
GGCGACAAATCGCGGCACATTCGAATTGAAATATTTCTTCACGGCCTCGATCGCCTCCGCCGACGGCGGCGAGGCGCATT
CCGCCGAGGCCGTGCGCCACCACATCAAGCAGCTGATCGATTCGGAAGCGCCGGCGGCGATCCTGTCGGATGACACCATC
GTGGAACGCTTACGCGCCTCGGGAATTGATATTGCCCGCCGCACGGTCGCGAAGTATCGCGAAGCCATGCGCATTCCCTC
GTCGGTACAACGCCGCCGTGACAAGCAGAGCGCTCTTGGTAACGTCCTCTCCACCGCAATGCCCGATCGCTCCCGCAACA
CCGAGCCGGCCTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(354-513)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(6-50)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(152-339)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

36.969

100

0.372