Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACK28Q_RS07515 Genome accession   NZ_CP176762
Coordinates   1630723..1632177 (+) Length   484 a.a.
NCBI ID   WP_011084688.1    Uniprot ID   P30332
Organism   Bradyrhizobium japonicum strain DSM 3014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1625723..1637177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK28Q_RS07505 (ACK28Q_07505) - 1625929..1626543 (+) 615 WP_011084684.1 J domain-containing protein -
  ACK28Q_RS07510 (ACK28Q_07510) - 1627644..1628429 (+) 786 WP_018645272.1 helix-turn-helix transcriptional regulator -
  ACK28Q_RS07515 (ACK28Q_07515) rpoN/rpoN1 1630723..1632177 (+) 1455 WP_011084688.1 RNA polymerase factor sigma-54 Machinery gene
  ACK28Q_RS07520 (ACK28Q_07520) - 1632862..1633137 (-) 276 WP_011084689.1 hypothetical protein -
  ACK28Q_RS07525 (ACK28Q_07525) - 1633153..1633200 (-) 48 Protein_1494 hypothetical protein -
  ACK28Q_RS07530 (ACK28Q_07530) - 1633273..1633443 (+) 171 WP_014497906.1 hypothetical protein -
  ACK28Q_RS07535 (ACK28Q_07535) - 1634001..1634345 (+) 345 WP_014497905.1 hypothetical protein -
  ACK28Q_RS07540 (ACK28Q_07540) - 1634342..1634548 (+) 207 WP_028170465.1 hypothetical protein -
  ACK28Q_RS07545 (ACK28Q_07545) - 1634672..1634995 (-) 324 WP_014497904.1 hypothetical protein -
  ACK28Q_RS07550 (ACK28Q_07550) - 1635984..1637015 (+) 1032 WP_038936655.1 TlpA disulfide reductase family protein -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 53772.80 Da        Isoelectric Point: 4.7595

>NTDB_id=968729 ACK28Q_RS07515 WP_011084688.1 1630723..1632177(+) (rpoN/rpoN1) [Bradyrhizobium japonicum strain DSM 3014]
MALTQRLEFRQSQSLVMSPQLMQAIKLLQLSNLDLMTFVEEELECNPLLERASDDAAGAEAPTEVDQVSGDQLAEAQVRD
ARDGAMTTYTEWGGGGSGDEDYNLEAFVASETTLSDHLAEQLSVAFTAPAQRMIGQYLIDLVDEAGYLPPDLGQAAERLG
ATQEDVEHVLAVLQEFDPPGVCARNLRECLAIQLRELDRYDPAMQALVEHLDLLAKRDIASLRKLCGVDDEDIADMIDEL
RRLSPKPGMKFGSARLQTMVPDVYVRPAPDGGWHVELNSDTLPRVLVNQTYYSKLSKKIGKDVDKSYFNDALQNATWLVR
ALDQRARTILKVATEIVRQQDGFFTLGVAHLRPLNLKAVAEAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIPSAD
GGEAHSAEAVRHRIKQLIESEEPSAVLSDDAIVERLRVSGIDIARRTVAKYREAMRIRSSVQRRRDNMWSTMNSRASGGT
GLDK

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=968729 ACK28Q_RS07515 WP_011084688.1 1630723..1632177(+) (rpoN/rpoN1) [Bradyrhizobium japonicum strain DSM 3014]
ATGGCGCTCACGCAAAGATTAGAGTTCCGGCAATCCCAGTCGCTGGTCATGTCGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAATTATCTAATCTCGACCTCATGACCTTCGTGGAGGAAGAGCTGGAGTGTAATCCGCTGCTCGAGCGTGCCAGTG
ACGATGCAGCTGGGGCCGAAGCCCCGACTGAGGTCGATCAGGTCAGCGGCGATCAGCTGGCCGAGGCCCAAGTGCGCGAC
GCCCGGGATGGCGCCATGACCACCTATACCGAATGGGGTGGCGGCGGCTCGGGTGACGAAGACTACAATCTCGAAGCGTT
TGTCGCGTCCGAGACAACATTGTCCGACCACCTGGCCGAACAACTGTCGGTCGCATTCACCGCGCCGGCGCAGCGCATGA
TCGGGCAGTATCTGATCGATCTCGTCGACGAAGCCGGCTATCTGCCGCCGGATCTCGGCCAGGCCGCCGAGCGGCTCGGC
GCAACGCAGGAGGATGTCGAGCACGTTCTTGCCGTCCTGCAGGAGTTCGATCCGCCCGGCGTCTGTGCGCGTAACTTGCG
CGAGTGCCTGGCGATCCAGCTCCGCGAGCTCGATAGATACGATCCGGCGATGCAGGCCCTCGTCGAGCATCTCGATCTCC
TCGCCAAGCGCGACATCGCGAGCTTGCGCAAGCTCTGCGGCGTCGACGACGAGGACATCGCCGACATGATCGACGAGCTC
CGCCGGCTCAGTCCCAAGCCGGGCATGAAGTTCGGGTCGGCGCGGCTGCAGACGATGGTACCCGACGTCTATGTCCGTCC
GGCTCCTGATGGCGGCTGGCATGTCGAGCTCAACAGCGACACCTTGCCGCGCGTGCTGGTCAACCAGACCTATTATTCCA
AGCTGTCGAAGAAGATCGGCAAGGACGTCGATAAGTCCTACTTCAACGACGCGCTGCAGAACGCGACCTGGCTGGTGCGC
GCGCTCGACCAGCGCGCCCGCACCATCCTGAAAGTTGCGACCGAGATCGTGCGTCAGCAGGACGGCTTCTTTACCCTTGG
TGTTGCGCATTTGCGGCCGCTGAATCTAAAGGCCGTTGCCGAGGCCATCCAGATGCATGAATCCACGGTGTCGCGCGTCA
CCGCCAACAAATACATGGCAACAAATCGCGGCACATTCGAGTTGAAATATTTCTTCACGGCATCGATCCCTTCGGCGGAT
GGCGGTGAGGCGCATTCCGCTGAAGCGGTGCGTCACCGCATCAAGCAGCTGATCGAATCCGAAGAGCCGTCAGCGGTTCT
GTCCGATGACGCGATCGTTGAGCGCCTGCGAGTCTCGGGCATTGATATTGCCCGCCGCACGGTCGCGAAGTACCGCGAAG
CCATGCGCATTCGGTCCTCGGTGCAGCGCCGCCGCGACAATATGTGGTCAACGATGAACAGTAGAGCATCGGGCGGAACT
GGCCTCGATAAATAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(6-50)

Sigma-54, DNA binding domain (Sigma54_DBD)

(306-465)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(104-291)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P30332

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

38.337

100

0.39

Multiple sequence alignment