Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MHH68_RS05315 Genome accession   NZ_CP150266
Coordinates   1089611..1093309 (+) Length   1232 a.a.
NCBI ID   WP_339165443.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-2841     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1084611..1098309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH68_RS05300 (MHH68_05300) - 1084907..1085317 (+) 411 WP_058014363.1 DoxX family protein -
  MHH68_RS05305 (MHH68_05305) - 1085513..1085959 (-) 447 WP_061407295.1 ferritin-like domain-containing protein -
  MHH68_RS05310 (MHH68_05310) addB 1086124..1089633 (+) 3510 WP_339165441.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHH68_RS05315 (MHH68_05315) addA 1089611..1093309 (+) 3699 WP_339165443.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHH68_RS05320 (MHH68_05320) sbcD 1093336..1094511 (+) 1176 WP_058014359.1 exonuclease subunit SbcD -
  MHH68_RS05325 (MHH68_05325) - 1094508..1097903 (+) 3396 WP_339165445.1 SMC family ATPase -
  MHH68_RS05330 (MHH68_05330) - 1097921..1098220 (+) 300 WP_017360217.1 hypothetical protein -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 142147.13 Da        Isoelectric Point: 5.0191

>NTDB_id=968712 MHH68_RS05315 WP_339165443.1 1089611..1093309(+) (addA) [Bacillus sp. FSL K6-2841]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPELPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQFLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMASVLEEAGDVVLSHPSTFTISFTQASDL
LTEDMSLEKKEQDEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESQIGHLLESLQQRDLLTDEQIRSINQEGIASFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYRHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAESILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=968712 MHH68_RS05315 WP_339165443.1 1089611..1093309(+) (addA) [Bacillus sp. FSL K6-2841]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCGGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACTTC
CAGTTGATGTGGACCGTCTGCTTGTTGTAACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCCG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGACCGTTACACGTCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCAAGATCGCATCCATCCCCTGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGTCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGTGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTATTAGAAAAAGCCACAGATGCACGTAATCAAGCGAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTTGAAAGAGCGAAGCAGGAAAAGTCAATTGTTGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATTGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAGGATACAAACCTTGTACAAGAAACCATTTTAAAGCTTGTATCTAAAGGAGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTGAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAACTTACAGTATCGTGACAT
TGTCATTTTGCTTCGATCTATGCCGTGGGCTCCACAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCCA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAATTGGCTCTGATTCGAACAAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTTATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAGCTAG
AGCGATTTTTCCAATTCCTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGACACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
CAGGGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAACTAGGGTTTGGAAGCAAGTACATCCATCCTGAACTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGAGTCCTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGTAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
TCCTGATTTTGAACGGTATCAATCAAAAACATACTTAGATTTCATTGGACCTGCCCTCATCCGTCATCAAGCGATGGCAT
CTGTTTTAGAAGAAGCAGGAGATGTTGTTCTTTCACATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAACGGAAGATATGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCAGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCTTATGATGAATCACAGATAGGACACTTGCTCGAATCTTTACAGCAGC
GAGATTTGCTAACAGATGAGCAGATCCGGTCAATTAATCAAGAAGGAATTGCATCTTTCTTCTCTACTTCAATTGGCCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCTTTTAGTATGGTGTTACCTGTTAAAGAGGTATACCGTCATAT
AGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAATCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCATTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTCGATGGGAA
TTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1224)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.262

100

0.665

Multiple sequence alignment