Detailed information NTG0968580

Bioinformatically predicted

Overview

• Name: clpE
• Type: Regulator
• Locus tag: ACK2VV_RS12615
• Genome accession: NZ_CP176712
• Coordinates: 2543791..2545896 (+)
• Length: 701 a.a.
• NCBI ID: WP_001058993.1
• Uniprot ID: Q99R88
• Organism: Staphylococcus aureus strain GTVSS-008
• Function: repress competence development (at the early growth phase) (predicted from homology) Competence regulation

Genomic Context

Location: 2538791..2550896

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK2VV_RS12590 (ACK2VV_12590)	-	2538820..2539038 (+)	219	WP_000011687.1	sterile alpha motif-like domain-containing protein	-
ACK2VV_RS12595 (ACK2VV_12595)	-	2539428..2539859 (+)	432	WP_000455861.1	CHAP domain-containing protein	-
ACK2VV_RS12600 (ACK2VV_12600)	-	2539998..2541275 (-)	1278	WP_001190998.1	hydroxymethylglutaryl-CoA reductase, degradative	-
ACK2VV_RS12605 (ACK2VV_12605)	-	2541530..2542696 (+)	1167	WP_000151982.1	hydroxymethylglutaryl-CoA synthase	-
ACK2VV_RS12610 (ACK2VV_12610)	-	2542861..2543382 (-)	522	WP_000455988.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
ACK2VV_RS12615 (ACK2VV_12615)	clpE	2543791..2545896 (+)	2106	WP_001058993.1	ATP-dependent Clp protease ATP-binding subunit ClpL	Regulator
ACK2VV_RS12620 (ACK2VV_12620)	-	2545965..2546135 (-)	171	WP_000111117.1	FeoB-associated Cys-rich membrane protein	-
ACK2VV_RS12625 (ACK2VV_12625)	feoB	2546148..2548142 (-)	1995	WP_049311749.1	ferrous iron transport protein B	-
ACK2VV_RS12630 (ACK2VV_12630)	-	2548154..2548381 (-)	228	WP_000932073.1	FeoA family protein	-

Sequence

Protein

• Length: 701 a.a.
• Molecular weight: 77857.23 Da
• Isoelectric Point: 4.5828

>NTDB_id=968580 ACK2VV_RS12615 WP_001058993.1 2543791..2545896(+) (clpE) [Staphylococcus aureus strain GTVSS-008]
MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEELAQLTQQGSNQSAEQSAQAFQQAAQRQQGQQGGNGNY
LEQIGRNLTQEARDGLLDPVIGRDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLSDILKPALSRGEISIIGATTQDE
YRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIREKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITA
AHLSAQSPAVDKVETEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTIQRLTGIPV
SQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPIGSFLFVGPTGVGKTELAKQLAIDLFGNKDA
LIRLDMSEYSDTTAVSKMIGTTAGYVGYDDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVI
NFKNTIIICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLLDDVQVTLDKKGITMDV
SQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHTDVKHVDIDVEDNELVVKGK

Nucleotide

• Length: 2106 bp

>NTDB_id=968580 ACK2VV_RS12615 WP_001058993.1 2543791..2545896(+) (clpE) [Staphylococcus aureus strain GTVSS-008]
ATGAATAACGGTTTTTTCAATAGCGACTTTGATTCAATTTTTCGAAGAATGATGCAAGATATGCAAGGTTCAAATCAAGT
CGGTAACAAAAAGTACTATATTAATGGTAAAGAAGTTTCACCTGAAGAACTAGCGCAACTCACACAACAAGGTAGCAATC
AATCTGCTGAACAAAGTGCGCAAGCTTTTCAACAAGCAGCACAAAGACAACAAGGGCAACAAGGTGGCAACGGCAATTAT
TTAGAACAAATTGGTCGTAACCTTACGCAAGAAGCACGTGACGGTTTATTAGATCCAGTCATTGGTCGTGATAAAGAAAT
TCAAGAAACTGCTGAAGTCTTAAGTAGACGAACTAAAAACAATCCTATATTAGTTGGAGAAGCTGGTGTTGGTAAAACTG
CGATTGTTGAAGGTTTAGCACAGGCAATCGTTGAAGGAAATGTACCAGCAGCAATCAAAGACAAAGAAATTATTTCTGTA
GATATTTCATCATTAGAAGCTGGAACGCAATATCGTGGTGCTTTTGAAGAAAATATTCAAAAATTAATCGAAGGTGTTAA
ATCTTCACAAAATGCCGTACTATTCTTTGATGAAATCCATCAAATTATCGGTTCAGGTGCCACAGGAAGTGATTCAGGTA
GCAAAGGGTTATCTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAA
TATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGA
TACTGTTGAAATTTTAAAAGGTATTCGCGAAAAATTCGAAGAACACCATCAAGTAAAATTACCAGATGACGTATTAAAAG
CATGTGTTGACTTATCAATTCAATATATTCCACAACGATTATTACCAGATAAAGCAATCGATGTGTTAGATATTACAGCA
GCACATTTATCTGCGCAAAGTCCAGCTGTCGATAAAGTTGAAACTGAAAAACGAATTTCTGAATTAGAAAATGATAAACG
TAAAGCAGTAAGTGCTGAAGAATATAAAAAAGCTGACGACATTCAAAATGAAATCAAATCATTACAAGATAAATTAGAAA
ACAGTAATGGTGAACATACTGCTGTTGCTACAGTTCATGATATTTCAGATACTATTCAACGATTAACTGGCATTCCAGTT
TCTCAAATGGATGATAACGATATTGAACGTTTAAAAAATATTTCTAATCGTTTAAGAAGTAAAATCATAGGTCAAGATCA
AGCTGTAGAAATGGTTTCACGTGCAATTCGCCGTAATCGCGCTGGCTTTGATGACGGCAACCGTCCAATTGGTAGTTTCT
TATTTGTCGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATCTATTTGGTAATAAAGATGCA
CTTATTCGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTCGG
TTATGATGACAATTCAAATACGTTAACTGAAAAAGTACGCCGTAATCCATACTCAGTCATTCTATTTGATGAAATCGAAA
AAGCAAATCCACAAATTTTAACATTGTTATTACAAGTAATGGATGATGGTAATTTGACTGATGGTCAAGGTAATGTCATC
AACTTTAAAAATACAATTATTATTTGTACATCAAATGCTGGCTTTGGCAATGGCAATGACGCTGAAGAAAAAGATATTAT
GCACGAAATGAAAAAATTCTTCCGCCCTGAATTCCTTAACCGCTTCAACGGCATCGTTGAATTCTTACATTTAGATAAAG
ATGCATTGCAAGATATCGTCAACTTATTATTAGACGATGTACAAGTTACATTAGATAAAAAAGGTATTACGATGGACGTT
TCTCAAGATGCGAAAGATTGGTTAATTGAAGAAGGCTATGATGAAGAATTAGGTGCACGTCCATTAAGACGTATTGTTGA
ACAGCAAGTACGTGACAAAATTACAGATTATTATCTAGATCATACAGACGTTAAACATGTGGATATAGATGTTGAGGATA
ACGAATTAGTCGTAAAAGGTAAATAA

Domains

Predicted by InterProScan.

• C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small) (611-682)

• AAA lid domain (AAA_lid_9) (264-363)

• ATPase family associated with various cellular activities (AAA) (AAA) (123-257)

• AAA domain (Cdc48 subfamily) (AAA_2) (448-603)

3D structure

• AlphaFold DB: Q99R88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
clpE	Streptococcus mutans UA159	48.834	91.726	0.448
clpC	Lactococcus lactis subsp. cremoris KW2	44.993	95.435	0.429
clpE	Streptococcus pneumoniae Rx1	48.298	88.017	0.425
clpE	Streptococcus pneumoniae D39	48.298	88.017	0.425
clpE	Streptococcus pneumoniae R6	48.298	88.017	0.425
clpE	Streptococcus pneumoniae TIGR4	48.298	88.017	0.425
clpC	Bacillus subtilis subsp. subtilis str. 168	43.658	96.719	0.422
clpC	Streptococcus pneumoniae Rx1	42.922	93.723	0.402
clpC	Streptococcus pneumoniae TIGR4	42.922	93.723	0.402
clpC	Streptococcus pneumoniae D39	42.922	93.723	0.402
clpC	Streptococcus thermophilus LMD-9	44.392	90.3	0.401
clpC	Streptococcus thermophilus LMG 18311	44.076	90.3	0.398
clpC	Streptococcus mutans UA159	43.577	87.732	0.382
clpC	Lactococcus lactis subsp. lactis strain DGCC12653	41.294	92.582	0.382