Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   MHH76_RS13320 Genome accession   NZ_CP150261
Coordinates   2678384..2679418 (-) Length   344 a.a.
NCBI ID   WP_035338372.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0193     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2673384..2684418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH76_RS13270 (MHH76_13270) - 2673454..2674248 (-) 795 WP_020452167.1 TasA family protein -
  MHH76_RS13275 (MHH76_13275) - 2674321..2674905 (-) 585 WP_020452168.1 signal peptidase I -
  MHH76_RS13280 (MHH76_13280) tapA 2674902..2675630 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  MHH76_RS13285 (MHH76_13285) - 2675908..2676228 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  MHH76_RS13290 (MHH76_13290) - 2676258..2676440 (-) 183 WP_020452171.1 YqzE family protein -
  MHH76_RS13295 (MHH76_13295) comGG 2676529..2676894 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  MHH76_RS13300 (MHH76_13300) comGF 2676906..2677394 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  MHH76_RS13305 (MHH76_13305) comGE 2677303..2677650 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  MHH76_RS13310 (MHH76_13310) comGD 2677634..2678071 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  MHH76_RS13315 (MHH76_13315) comGC 2678077..2678370 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  MHH76_RS13320 (MHH76_13320) comGB 2678384..2679418 (-) 1035 WP_035338372.1 competence type IV pilus assembly protein ComGB Machinery gene
  MHH76_RS13325 (MHH76_13325) comGA 2679408..2680472 (-) 1065 WP_025811157.1 competence type IV pilus ATPase ComGA Machinery gene
  MHH76_RS13330 (MHH76_13330) - 2680630..2681469 (-) 840 WP_035338373.1 STAS domain-containing protein -
  MHH76_RS13340 (MHH76_13340) - 2681711..2682088 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHH76_RS13345 (MHH76_13345) - 2682299..2682544 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  MHH76_RS13350 (MHH76_13350) - 2682580..2683218 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  MHH76_RS13355 (MHH76_13355) - 2683374..2683547 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  MHH76_RS13360 (MHH76_13360) - 2683608..2683922 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39807.12 Da        Isoelectric Point: 9.6575

>NTDB_id=968536 MHH76_RS13320 WP_035338372.1 2678384..2679418(-) (comGB) [Bacillus sp. FSL L8-0193]
MKIKMKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LSFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=968536 MHH76_RS13320 WP_035338372.1 2678384..2679418(-) (comGB) [Bacillus sp. FSL L8-0193]
ATGAAGATTAAAATGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCGTATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATCCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTATCATTTCATAAAAATGAAGCGAAGCGCCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACACTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538

Multiple sequence alignment