Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MHH76_RS13315 Genome accession   NZ_CP150261
Coordinates   2678077..2678370 (-) Length   97 a.a.
NCBI ID   WP_020452176.1    Uniprot ID   A0A6N2FHJ1
Organism   Bacillus sp. FSL L8-0193     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2673077..2683370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH76_RS13270 (MHH76_13270) - 2673454..2674248 (-) 795 WP_020452167.1 TasA family protein -
  MHH76_RS13275 (MHH76_13275) - 2674321..2674905 (-) 585 WP_020452168.1 signal peptidase I -
  MHH76_RS13280 (MHH76_13280) tapA 2674902..2675630 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  MHH76_RS13285 (MHH76_13285) - 2675908..2676228 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  MHH76_RS13290 (MHH76_13290) - 2676258..2676440 (-) 183 WP_020452171.1 YqzE family protein -
  MHH76_RS13295 (MHH76_13295) comGG 2676529..2676894 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  MHH76_RS13300 (MHH76_13300) comGF 2676906..2677394 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  MHH76_RS13305 (MHH76_13305) comGE 2677303..2677650 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  MHH76_RS13310 (MHH76_13310) comGD 2677634..2678071 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  MHH76_RS13315 (MHH76_13315) comGC 2678077..2678370 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  MHH76_RS13320 (MHH76_13320) comGB 2678384..2679418 (-) 1035 WP_035338372.1 competence type IV pilus assembly protein ComGB Machinery gene
  MHH76_RS13325 (MHH76_13325) comGA 2679408..2680472 (-) 1065 WP_025811157.1 competence type IV pilus ATPase ComGA Machinery gene
  MHH76_RS13330 (MHH76_13330) - 2680630..2681469 (-) 840 WP_035338373.1 STAS domain-containing protein -
  MHH76_RS13340 (MHH76_13340) - 2681711..2682088 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHH76_RS13345 (MHH76_13345) - 2682299..2682544 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  MHH76_RS13350 (MHH76_13350) - 2682580..2683218 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10670.54 Da        Isoelectric Point: 9.1200

>NTDB_id=968535 MHH76_RS13315 WP_020452176.1 2678077..2678370(-) (comGC) [Bacillus sp. FSL L8-0193]
MNEKGFTLIEMLVVMLVISILLLITIPNVTKHNQSIQKKGCEGLINMVQAQITAYQMDHDGKTPSRADLESEGYVKKNLK
CPNGKAIKISNGKAQAD

Nucleotide


Download         Length: 294 bp        

>NTDB_id=968535 MHH76_RS13315 WP_020452176.1 2678077..2678370(-) (comGC) [Bacillus sp. FSL L8-0193]
GTGAATGAAAAGGGATTTACACTGATAGAAATGTTAGTCGTCATGCTCGTCATCTCCATCCTGCTGTTGATCACCATACC
GAATGTGACCAAGCATAATCAAAGTATTCAAAAGAAAGGATGCGAAGGGTTAATTAATATGGTTCAAGCGCAAATCACGG
CTTATCAAATGGATCATGACGGGAAAACGCCGAGTAGGGCGGACCTTGAATCTGAAGGTTACGTGAAGAAGAACTTAAAG
TGCCCGAATGGGAAAGCAATTAAAATTTCAAACGGCAAAGCACAGGCTGATTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N2FHJ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

71.134

100

0.711
  comGC Staphylococcus aureus MW2

45.263

97.938

0.443
  comGC Staphylococcus aureus N315

45.263

97.938

0.443

Multiple sequence alignment