Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   MHH76_RS10110 Genome accession   NZ_CP150261
Coordinates   1994812..1995240 (+) Length   142 a.a.
NCBI ID   WP_020451590.1    Uniprot ID   A0A6N2GRD1
Organism   Bacillus sp. FSL L8-0193     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 1989812..2000240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH76_RS10080 (MHH76_10080) - 1990068..1990676 (+) 609 WP_023857599.1 flavin reductase family protein -
  MHH76_RS10085 (MHH76_10085) wrbA 1990878..1991492 (+) 615 WP_026579653.1 NAD(P)H:quinone oxidoreductase -
  MHH76_RS10090 (MHH76_10090) - 1991803..1991943 (+) 141 WP_023857597.1 hypothetical protein -
  MHH76_RS10095 (MHH76_10095) - 1991936..1992769 (+) 834 WP_020451587.1 prohibitin family protein -
  MHH76_RS10100 (MHH76_10100) - 1992858..1993619 (+) 762 WP_026579652.1 hypothetical protein -
  MHH76_RS10105 (MHH76_10105) - 1993679..1994518 (+) 840 WP_026579651.1 thymidylate synthase -
  MHH76_RS10110 (MHH76_10110) nucA/comI 1994812..1995240 (+) 429 WP_020451590.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHH76_RS10115 (MHH76_10115) lexA 1995439..1996062 (-) 624 WP_020451591.1 transcriptional repressor LexA -
  MHH76_RS10120 (MHH76_10120) yneA 1996214..1996525 (+) 312 WP_020451592.1 cell division suppressor protein YneA -
  MHH76_RS10125 (MHH76_10125) - 1996544..1997191 (+) 648 WP_020451593.1 recombinase family protein -
  MHH76_RS10130 (MHH76_10130) - 1997257..1997490 (+) 234 WP_020451594.1 DUF896 domain-containing protein -
  MHH76_RS10135 (MHH76_10135) tkt 1997654..1999657 (+) 2004 WP_023857593.1 transketolase -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15420.36 Da        Isoelectric Point: 7.8950

>NTDB_id=968529 MHH76_RS10110 WP_020451590.1 1994812..1995240(+) (nucA/comI) [Bacillus sp. FSL L8-0193]
MIKKWAVHLLFSALVLLGLSGGAAYSPQHAEGAARYDDVLYFPASRYPETGAHISDAIKAGHSDVCTIERSGADKRRQES
LKGIPTKPGFDRDEWPMAMCEEGGKGASVRYVSSSDNRGAGSWVGNRLSDYADGTRILFIVR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=968529 MHH76_RS10110 WP_020451590.1 1994812..1995240(+) (nucA/comI) [Bacillus sp. FSL L8-0193]
ATGATCAAAAAATGGGCGGTTCATCTGCTGTTTTCCGCATTGGTGCTGCTTGGGCTTTCGGGAGGGGCTGCATATTCTCC
TCAGCATGCCGAAGGCGCTGCAAGGTATGATGACGTATTGTATTTTCCGGCATCGCGCTATCCTGAAACAGGTGCTCATA
TAAGCGATGCGATCAAAGCGGGCCATTCAGATGTCTGCACAATTGAAAGATCTGGAGCGGATAAGCGCCGTCAGGAATCA
TTAAAGGGGATTCCGACTAAGCCGGGCTTTGACCGTGACGAATGGCCGATGGCCATGTGTGAAGAAGGGGGAAAAGGAGC
GTCGGTCAGATATGTCAGCTCATCGGATAACCGCGGAGCCGGCTCCTGGGTCGGGAACAGGCTGAGCGATTACGCTGACG
GGACGAGAATTTTGTTTATCGTTCGATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6N2GRD1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.524

88.732

0.528


Multiple sequence alignment