Detailed information NTG0968511

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: MHH76_RS06135
• Genome accession: NZ_CP150261
• Coordinates: 1237018..1240710 (+)
• Length: 1230 a.a.
• NCBI ID: WP_043054269.1
• Uniprot ID: -
• Organism: Bacillus sp. FSL L8-0193
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1232018..1245710

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MHH76_RS06125 (MHH76_06125)	modB	1232765..1233421 (+)	657	WP_035339021.1	molybdate ABC transporter permease subunit	-
MHH76_RS06130 (MHH76_06130)	addB	1233531..1237031 (+)	3501	WP_043054268.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MHH76_RS06135 (MHH76_06135)	addA	1237018..1240710 (+)	3693	WP_043054269.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MHH76_RS06140 (MHH76_06140)	sbcD	1240776..1241957 (+)	1182	WP_003180431.1	exonuclease subunit SbcD	-
MHH76_RS06145 (MHH76_06145)	sbcC	1241954..1245346 (+)	3393	WP_043054270.1	exonuclease subunit SbcC	-
MHH76_RS06150 (MHH76_06150)	-	1245391..1245609 (-)	219	WP_016885923.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141295.14 Da
• Isoelectric Point: 5.4195

>NTDB_id=968511 MHH76_RS06135 WP_043054269.1 1237018..1240710(+) (addA) [Bacillus sp. FSL L8-0193]
MEISKPKGSNWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQEHPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKQDPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHTFVDLYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQNDFSALYELVPAVSFQRLKPCKGDAYDPRLVDEAADLRNSAKKQLEKLK
SDYFTRTPEQHLESLREMKPVIQTLVQLVLEYGKRFADAKKEKAIVDFSDLEHDCLAILSGKNQAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKFVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPSNEATKTELLLIENQSGAQDEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDALLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRSQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPLVIDEIREHPARFQVSWLSAADLQAE
DIDQAGEERHGRLSQIQMGLPIDGSFEYEQQVRERLTWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPTKDAL
LFKRPSFMMVKGLTAAERGTAMHTVMQHIPLTRALEMNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=968511 MHH76_RS06135 WP_043054269.1 1237018..1240710(+) (addA) [Bacillus sp. FSL L8-0193]
ATGGAGATTTCTAAACCGAAGGGCAGCAATTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGCTCCGGAAAAACGGCCGTGCTGGTTGAGCGGATCATTCGGAAAATAACGGATCAGGAACATC
CCGTAGATGTCGACAGGCTGCTTGTCGTCACCTTTACGAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGTTCGCTTCATTTGCGCAGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCACTCATTCTGTCTTCAAGTCATCCGCAAATACTATTATTTGATCGATGTTGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTTGGCGATGAGGTGCTGGACGAGCTGTTTGAAGAAGAATACAAAAAGCAAGATCCGGCC
TTTTTTGAACTTGTGGACAGATATACGACAGACCGTCATGACCTCGATTTGCAGCATCTCGTCAAGCGGGTCTACGAGTT
TTCCCGTTCGCATCCCGATCCGGAAGGCTGGCTTCACACATTTGTTGATCTGTATGACGCGGCTTCAGATACGAAAGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAGGGGATGCGGCAAAAGCTGACGCGTGCG
CTTGATTTGACAAAGCAGCCAGGGGGGCCTGCGCCGCGCGCCGAAAATTTCCTCGATGATTTAGCGCAGATTGACAGGCT
GATTCAGCAGCAGAATGATTTTTCGGCTCTGTACGAGCTTGTACCGGCCGTTTCGTTTCAAAGATTGAAGCCGTGCAAAG
GAGATGCGTACGATCCTCGATTGGTTGATGAGGCCGCAGATCTCCGCAACAGCGCGAAAAAGCAACTTGAAAAGCTGAAA
AGCGATTATTTTACGCGAACTCCCGAGCAGCACCTTGAGAGCTTGAGGGAAATGAAGCCGGTCATTCAGACGCTCGTCCA
GCTCGTGTTGGAGTATGGCAAGCGGTTTGCGGACGCGAAAAAGGAAAAAGCGATCGTCGATTTCTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGGGAAAAACCAAGCCGGCGAAGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCATGAAGTGCTTGTTGATGAATATCAGGATACGAACTTGGTCCAGGAAGCGATTTTAAAGTTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCAGAACCGCTTTTGTTCC
TTTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGTCAAAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAGCAGCTCATGGGAGAAAAGGTCGGGGAAGTCGAATATGACGAACA
GGCCGAATTGAAGCTTGGCGCGTCTTATCCGTCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAAACCAGAGCG
GCGCGCAGGATGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGACAAATCCGCAGGCTG
ATAACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCACGGAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGATCGATGCCTTGGGCGCCGCAAATTATGGACGAGTTTAAACAGCAGGGGATTCCGGTATACGCGAACCTCTCAACGG
GTTATTTTGAAGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCTTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTAGGTCTTGACGAAAATGAGCTTTCCTTGATCAGAATCAAGGATAAAAAAGCGCC
GTTTTACGAAGCGATGAAAGCATACCTTGCTGCAGCAGATGGCGATGAGAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
CCCTGCTCAAAAAGTGGAGAGCGTATGCGAAAAACCACTCTGTGGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCAAGATC
CTATGAAGCGACATCGTTCCGCGGTTTGTTCCGCTTTTTAAGGTTTATTGAGCGGATGCAGGAGCGGGGTGATGATCTCG
GAACGGCAAGAGCTCTGAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAGTCTTATCTGCTGGATAAAGAACTCGG
TTTCGGAACGAAGTTTATTCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTGATCGCGATGAAGAAAAAGCTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCTCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCTAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGTCACAAGCGGCGGGGTCTGAGTGGCTGCTTCCGGATTATGA
ACGGTTTCAGGCGAAATCTTACCTGGATTTCATCGGACCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGCGCGC
CGCTGGTGATTGATGAGATCCGTGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCGGCCGCGGATCTTCAAGCAGAA
GATATTGATCAGGCCGGTGAAGAAAGACACGGCCGGCTCTCCCAAATTCAAATGGGGCTTCCGATCGATGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAAAGGCTGACATGGTCATATCCATACAAAGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGGCAGAAGGAGTATGAGGATGAGTACGGCGACCGCTCGCTGATTCGCCCAACCAAGGATGCTCTC
CTGTTTAAACGGCCGTCCTTTATGATGGTTAAAGGCCTGACCGCGGCCGAACGAGGGACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTGCGCTTGAAATGAATGAGCTCAGCCGCCTCCTAGACAGGCTTGTTGAGAAAGAGCTATTAA
CAGAAGACCAGCGGGCAGCGATTGAGGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGC
GCCCGCCGCGTCGAACGGGAAGTTCCCTTTAACATGACGCTGTCCGCCAAAGAGGTTTATCCTGATCTTGAGTCAGCCGA
CGAACCTGTATTGATACAGGGAATTATCGATTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACCG
ACCGGATACACGGAAAGTACCGAAACGGATTCGAAGGGGCCGAACCGATTTTAAGGAAGCGTTACGAAACACAAATTCAG
CTTTATGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCACTGTACTTTTTTGACGGAGGACATGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1222)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	72.055	100	0.721