Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACK2VU_RS03295 Genome accession   NZ_CP176687
Coordinates   725097..726479 (+) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli O157:H7 strain GTVSS-023     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 720097..731479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2VU_RS03270 (ACK2VU_03270) deoD 721111..721830 (+) 720 WP_000224877.1 purine-nucleoside phosphorylase -
  ACK2VU_RS03275 (ACK2VU_03275) - 721991..722254 (-) 264 WP_000566150.1 helix-turn-helix domain-containing protein -
  ACK2VU_RS03280 (ACK2VU_03280) lplA 722286..723302 (-) 1017 WP_000105832.1 lipoate--protein ligase LplA -
  ACK2VU_RS03285 (ACK2VU_03285) ytjB 723333..723974 (-) 642 Protein_622 YtjB family periplasmic protein -
  ACK2VU_RS03290 (ACK2VU_03290) serB 724080..725048 (+) 969 WP_001132955.1 phosphoserine phosphatase -
  ACK2VU_RS03295 (ACK2VU_03295) radA/sms 725097..726479 (+) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  ACK2VU_RS03300 (ACK2VU_03300) nadR 726500..727732 (+) 1233 WP_000093817.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACK2VU_RS03305 (ACK2VU_03305) nadS 727766..727879 (+) 114 Protein_626 NadS family protein -
  ACK2VU_RS03310 (ACK2VU_03310) ettA 728040..729707 (-) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=968435 ACK2VU_RS03295 WP_001029698.1 725097..726479(+) (radA/sms) [Escherichia coli O157:H7 strain GTVSS-023]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=968435 ACK2VU_RS03295 WP_001029698.1 725097..726479(+) (radA/sms) [Escherichia coli O157:H7 strain GTVSS-023]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCTACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTAGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAG
TGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTAAAGGTAACCGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43

Multiple sequence alignment