Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   ACK2VH_RS27455 Genome accession   NZ_CP176684
Coordinates   5968748..5969113 (-) Length   121 a.a.
NCBI ID   WP_003084587.1    Uniprot ID   A0A0H2ZKW2
Organism   Pseudomonas aeruginosa strain GTVSS-024     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 5963748..5974113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2VH_RS27440 (ACK2VH_27435) pilK 5965092..5965967 (-) 876 WP_003100938.1 type 4 fimbrial methyltransferase PilK -
  ACK2VH_RS27445 (ACK2VH_27440) pilJ 5966028..5968076 (-) 2049 WP_003100940.1 chemotaxis chemoreceptor PilJ -
  ACK2VH_RS27450 (ACK2VH_27445) - 5968161..5968697 (-) 537 WP_003084590.1 chemotaxis protein CheW -
  ACK2VH_RS27455 (ACK2VH_27450) pilH 5968748..5969113 (-) 366 WP_003084587.1 twitching motility response regulator PilH Machinery gene
  ACK2VH_RS27460 (ACK2VH_27455) pilG 5969160..5969567 (-) 408 WP_003084583.1 twitching motility response regulator PilG Regulator
  ACK2VH_RS27465 (ACK2VH_27460) gshB 5969822..5970775 (+) 954 WP_042853546.1 glutathione synthase -
  ACK2VH_RS27470 (ACK2VH_27465) - 5970922..5971815 (+) 894 WP_003160914.1 energy transducer TonB -
  ACK2VH_RS27475 (ACK2VH_27470) - 5971864..5972433 (+) 570 WP_003100947.1 YqgE/AlgH family protein -
  ACK2VH_RS27480 (ACK2VH_27475) ruvX 5972433..5972867 (+) 435 WP_003121753.1 Holliday junction resolvase RuvX -
  ACK2VH_RS27485 (ACK2VH_27480) pyrR 5972979..5973491 (+) 513 WP_003084574.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence


Protein


Download         Length: 121 a.a.        Molecular weight: 13273.30 Da        Isoelectric Point: 5.1961

>NTDB_id=968408 ACK2VH_RS27455 WP_003084587.1 5968748..5969113(-) (pilH) [Pseudomonas aeruginosa strain GTVSS-024]
MARILIVDDSPTEMYKLTAMLEKHGHQVLKAENGGDGVALARQEKPDVVLMDIVMPGLNGFQATRQLTKDAETSAIPVII
VTTKDQETDKVWGKRQGARDYLTKPVDEETLLKTINAVLAG

Nucleotide


Download         Length: 366 bp        

>NTDB_id=968408 ACK2VH_RS27455 WP_003084587.1 5968748..5969113(-) (pilH) [Pseudomonas aeruginosa strain GTVSS-024]
ATGGCTCGTATTTTGATTGTTGATGACTCTCCGACCGAGATGTACAAGCTGACCGCCATGCTGGAAAAGCATGGTCACCA
GGTACTCAAGGCCGAGAACGGCGGCGACGGCGTCGCCCTGGCCCGCCAGGAAAAGCCCGACGTGGTCCTGATGGACATCG
TCATGCCCGGCCTCAACGGCTTCCAGGCGACCCGTCAATTGACCAAGGACGCCGAGACCAGCGCCATCCCGGTGATCATC
GTCACCACCAAGGACCAGGAGACCGACAAGGTCTGGGGCAAGCGCCAGGGCGCTCGCGACTACCTGACCAAGCCGGTGGA
CGAAGAGACCCTGCTGAAAACCATCAATGCGGTGCTGGCGGGCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0H2ZKW2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

36.975

98.347

0.364


Multiple sequence alignment