Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MKY32_RS14635 Genome accession   NZ_CP150259
Coordinates   2769081..2769710 (-) Length   209 a.a.
NCBI ID   WP_003183693.1    Uniprot ID   Q8VQ77
Organism   Bacillus sp. FSL K6-0998     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2764081..2774710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY32_RS14610 (MKY32_14610) rpsT 2764295..2764561 (+) 267 WP_003183682.1 30S ribosomal protein S20 -
  MKY32_RS14615 (MKY32_14615) holA 2764623..2765666 (-) 1044 WP_009327866.1 DNA polymerase III subunit delta -
  MKY32_RS14620 (MKY32_14620) - 2765896..2766027 (+) 132 WP_003183686.1 YqzM family protein -
  MKY32_RS14625 (MKY32_14625) - 2766052..2768439 (-) 2388 WP_009327865.1 DNA internalization-related competence protein ComEC/Rec2 -
  MKY32_RS14630 (MKY32_14630) - 2768443..2769012 (-) 570 WP_003183690.1 ComE operon protein 2 -
  MKY32_RS14635 (MKY32_14635) comEA 2769081..2769710 (-) 630 WP_003183693.1 helix-hairpin-helix domain-containing protein Machinery gene
  MKY32_RS14640 (MKY32_14640) comER 2769794..2770615 (+) 822 WP_003183696.1 late competence protein ComER -
  MKY32_RS14645 (MKY32_14645) - 2770685..2771440 (-) 756 WP_003183697.1 class I SAM-dependent methyltransferase -
  MKY32_RS14650 (MKY32_14650) rsfS 2771437..2771793 (-) 357 WP_025808063.1 ribosome silencing factor -
  MKY32_RS14655 (MKY32_14655) yqeK 2771807..2772370 (-) 564 WP_003183701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MKY32_RS14660 (MKY32_14660) - 2772360..2772929 (-) 570 WP_003183702.1 nicotinate-nucleotide adenylyltransferase -
  MKY32_RS14665 (MKY32_14665) yhbY 2772948..2773238 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  MKY32_RS14670 (MKY32_14670) aroE 2773232..2774068 (-) 837 WP_003183706.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22468.42 Da        Isoelectric Point: 5.1890

>NTDB_id=968394 MKY32_RS14635 WP_003183693.1 2769081..2769710(-) (comEA) [Bacillus sp. FSL K6-0998]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTYDKKEEVKREKSAGKEAVIIDLKGAVKNPGV
YQMKEGDRVHDVLKKAGGTEKKADQKQINLAAVLQDGMVVYIPFEGEEAADSFSKAGSRADGASVDIVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFHTVEDITNVSGIGEKSFERIKSAITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=968394 MKY32_RS14635 WP_003183693.1 2769081..2769710(-) (comEA) [Bacillus sp. FSL K6-0998]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCTGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCCGAGGAGGCTTCTGCACAGACGTATGACAAGAAGG
AGGAGGTGAAACGGGAAAAAAGCGCGGGAAAAGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAAGAGGGAGACAGGGTCCACGATGTATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGATCAAAAGCA
AATTAACCTTGCAGCCGTTTTGCAGGACGGTATGGTGGTTTACATTCCATTTGAGGGGGAAGAGGCTGCTGATTCTTTCT
CGAAAGCGGGTTCAAGGGCAGATGGCGCTTCCGTCGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCCGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGCGAGGAGAACGGACCGTTTCACACGGTAGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTGCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(147-207)

SLBB domain (SLBB)

(69-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526

Multiple sequence alignment