Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MKY32_RS14465 Genome accession   NZ_CP150259
Coordinates   2736489..2737259 (-) Length   256 a.a.
NCBI ID   WP_016885480.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0998     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2731489..2742259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY32_RS14445 (MKY32_14445) - 2731563..2732375 (-) 813 WP_003183615.1 pyruvate, water dikinase regulatory protein -
  MKY32_RS14450 (MKY32_14450) - 2732401..2733039 (-) 639 WP_025804968.1 helix-turn-helix transcriptional regulator -
  MKY32_RS14455 (MKY32_14455) glyS 2733237..2735276 (-) 2040 WP_009327872.1 glycine--tRNA ligase subunit beta -
  MKY32_RS14460 (MKY32_14460) glyQ 2735269..2736156 (-) 888 WP_003183621.1 glycine--tRNA ligase subunit alpha -
  MKY32_RS14465 (MKY32_14465) recO 2736489..2737259 (-) 771 WP_016885480.1 DNA repair protein RecO Machinery gene
  MKY32_RS14470 (MKY32_14470) - 2737297..2737440 (-) 144 WP_003183626.1 YqzL family protein -
  MKY32_RS14475 (MKY32_14475) era 2737602..2738507 (-) 906 WP_009327871.1 GTPase Era -
  MKY32_RS14480 (MKY32_14480) - 2738488..2738898 (-) 411 WP_003183631.1 cytidine deaminase -
  MKY32_RS14485 (MKY32_14485) - 2739030..2739410 (-) 381 WP_003183633.1 diacylglycerol kinase family protein -
  MKY32_RS14490 (MKY32_14490) ybeY 2739388..2739864 (-) 477 WP_003183635.1 rRNA maturation RNase YbeY -
  MKY32_RS14495 (MKY32_14495) - 2739861..2741915 (-) 2055 WP_237562993.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29531.16 Da        Isoelectric Point: 8.3083

>NTDB_id=968392 MKY32_RS14465 WP_016885480.1 2736489..2737259(-) (recO) [Bacillus sp. FSL K6-0998]
MLTKCEGIVLRTNDYGETNKIITLLTREHGKIGVLARGAKKPNSRLSAISQPFLYASFLFRKSSGLGTLEQGEMIESMRN
IREDLFLTAYAAYIAELIDKGTEEKKPNPFLFELLLQSMRRLNDGTDPDIIANLIEVKMLSVMGLYPELDECVHCKSKDG
TFHFSIRDNGFICHRCFAKDPYRIPLSPASARLLRLFFYFDLSRLGNVSVKQETKDELKRVIQTYYDEYSGIYLKSRRFM
EQMDNMKKMLDDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=968392 MKY32_RS14465 WP_016885480.1 2736489..2737259(-) (recO) [Bacillus sp. FSL K6-0998]
ATGCTGACGAAATGTGAAGGTATCGTCCTTCGTACAAACGACTATGGTGAAACAAATAAGATCATTACGCTATTGACGAG
AGAACACGGGAAAATCGGTGTGCTGGCAAGGGGCGCCAAAAAGCCGAACAGCCGCTTGTCAGCGATTTCCCAGCCGTTCC
TTTATGCATCTTTTTTATTCCGCAAATCATCCGGCCTCGGCACATTGGAGCAGGGCGAGATGATCGAAAGCATGAGGAAT
ATCCGCGAGGATCTGTTTTTAACAGCTTACGCGGCCTACATCGCTGAATTAATCGACAAGGGAACGGAGGAAAAGAAACC
GAATCCGTTTTTATTTGAGCTGCTGCTTCAGTCAATGAGGAGGCTGAATGACGGTACAGATCCCGACATTATCGCCAACC
TCATCGAAGTCAAAATGCTCAGCGTGATGGGGCTGTATCCGGAGCTGGATGAATGTGTGCACTGCAAAAGCAAAGACGGC
ACATTTCATTTTTCGATCCGCGACAACGGCTTTATCTGCCACCGCTGTTTTGCGAAAGATCCATACAGGATACCGCTGTC
TCCCGCATCCGCCAGGCTGTTGAGACTGTTTTTTTATTTTGACCTGTCAAGGCTCGGAAATGTCTCGGTCAAACAGGAGA
CGAAGGATGAGCTGAAAAGGGTTATTCAGACGTACTATGACGAGTACTCAGGGATCTACCTGAAATCGCGGCGCTTCATG
GAACAAATGGACAATATGAAAAAGATGCTGGACGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

77.734

100

0.777

Multiple sequence alignment