Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | MKX38_RS12665 | Genome accession | NZ_CP150258 |
| Coordinates | 2467678..2468088 (-) | Length | 136 a.a. |
| NCBI ID | WP_227057745.1 | Uniprot ID | - |
| Organism | Bacillus sp. FSL M8-0025 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2452834..2488006 | 2467678..2468088 | within | 0 |
Gene organization within MGE regions
Location: 2452834..2488006
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKX38_RS12575 (MKX38_12575) | comEC | 2452834..2455164 (-) | 2331 | WP_128993168.1 | DNA internalization-related competence protein ComEC/Rec2 | Machinery gene |
| MKX38_RS12580 (MKX38_12580) | - | 2455168..2455737 (-) | 570 | WP_003229978.1 | ComE operon protein 2 | - |
| MKX38_RS12585 (MKX38_12585) | comEA | 2455804..2456421 (-) | 618 | WP_032722140.1 | competence protein ComEA | Machinery gene |
| MKX38_RS12590 (MKX38_12590) | comER | 2456505..2457326 (+) | 822 | WP_032722142.1 | late competence protein ComER | - |
| MKX38_RS12595 (MKX38_12595) | - | 2457392..2458135 (-) | 744 | WP_339187283.1 | class I SAM-dependent methyltransferase | - |
| MKX38_RS12600 (MKX38_12600) | rsfS | 2458132..2458488 (-) | 357 | WP_041333759.1 | ribosome silencing factor | - |
| MKX38_RS12605 (MKX38_12605) | yqeK | 2458506..2459066 (-) | 561 | WP_014480316.1 | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK | - |
| MKX38_RS12610 (MKX38_12610) | - | 2459056..2459625 (-) | 570 | WP_004398676.1 | nicotinate-nucleotide adenylyltransferase | - |
| MKX38_RS12615 (MKX38_12615) | yhbY | 2459637..2459927 (-) | 291 | WP_003226133.1 | ribosome assembly RNA-binding protein YhbY | - |
| MKX38_RS12620 (MKX38_12620) | aroE | 2459921..2460763 (-) | 843 | WP_003229967.1 | shikimate dehydrogenase | - |
| MKX38_RS12625 (MKX38_12625) | yqeH | 2460781..2461881 (-) | 1101 | WP_003229966.1 | ribosome biogenesis GTPase YqeH | - |
| MKX38_RS12630 (MKX38_12630) | - | 2461885..2462403 (-) | 519 | WP_105990408.1 | YqeG family HAD IIIA-type phosphatase | - |
| MKX38_RS12635 (MKX38_12635) | - | 2462765..2462905 (+) | 141 | WP_003226124.1 | sporulation histidine kinase inhibitor Sda | - |
| MKX38_RS12640 (MKX38_12640) | - | 2463211..2463942 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| MKX38_RS12645 (MKX38_12645) | cwlH | 2464194..2464946 (-) | 753 | WP_163138266.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| MKX38_RS12650 (MKX38_12650) | - | 2465133..2465759 (+) | 627 | WP_038429330.1 | TVP38/TMEM64 family protein | - |
| MKX38_RS12655 (MKX38_12655) | gnd | 2465778..2466671 (-) | 894 | WP_339187289.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| MKX38_RS12660 (MKX38_12660) | - | 2466923..2467645 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| MKX38_RS12665 (MKX38_12665) | nucA/comI | 2467678..2468088 (-) | 411 | WP_227057745.1 | sporulation-specific Dnase NucB | Machinery gene |
| MKX38_RS12670 (MKX38_12670) | - | 2468284..2468628 (+) | 345 | Protein_2445 | sigma-70 family RNA polymerase sigma factor | - |
| MKX38_RS12675 (MKX38_12675) | - | 2468774..2469244 (+) | 471 | WP_033884465.1 | MarR family transcriptional regulator | - |
| MKX38_RS12680 (MKX38_12680) | - | 2469395..2470807 (+) | 1413 | WP_339187293.1 | MDR family MFS transporter | - |
| MKX38_RS12685 (MKX38_12685) | spoIVCA | 2470994..2472454 (-) | 1461 | WP_235701260.1 | site-specific DNA recombinase SpoIVCA | - |
| MKX38_RS12690 (MKX38_12690) | - | 2472412..2472590 (-) | 179 | Protein_2449 | hypothetical protein | - |
| MKX38_RS12695 (MKX38_12695) | - | 2472999..2473721 (-) | 723 | WP_119899593.1 | NAD(P)-binding domain-containing protein | - |
| MKX38_RS12700 (MKX38_12700) | - | 2473947..2474306 (+) | 360 | WP_049635841.1 | winged helix-turn-helix transcriptional regulator | - |
| MKX38_RS12705 (MKX38_12705) | nfsA | 2474786..2475526 (+) | 741 | WP_119899595.1 | oxygen-insensitive NADPH nitroreductase | - |
| MKX38_RS12710 (MKX38_12710) | - | 2476004..2477131 (-) | 1128 | WP_038429342.1 | Rap family tetratricopeptide repeat protein | - |
| MKX38_RS12715 (MKX38_12715) | - | 2477318..2479210 (+) | 1893 | WP_339187298.1 | T7SS effector LXG polymorphic toxin | - |
| MKX38_RS12720 (MKX38_12720) | cdiI | 2479232..2479591 (+) | 360 | WP_040082462.1 | ribonuclease toxin immunity protein CdiI | - |
| MKX38_RS12725 (MKX38_12725) | - | 2479643..2479978 (+) | 336 | WP_317680597.1 | tRNA-Val4 | - |
| MKX38_RS12730 (MKX38_12730) | - | 2480039..2480479 (+) | 441 | WP_317680596.1 | SMI1/KNR4 family protein | - |
| MKX38_RS12735 (MKX38_12735) | - | 2480673..2481218 (-) | 546 | WP_268310042.1 | SMI1/KNR4 family protein | - |
| MKX38_RS12740 (MKX38_12740) | - | 2481239..2482231 (-) | 993 | WP_317680595.1 | HNH endonuclease signature motif containing protein | - |
| MKX38_RS12745 (MKX38_12745) | - | 2482364..2483322 (-) | 959 | Protein_2460 | N-acetylmuramoyl-L-alanine amidase | - |
| MKX38_RS12750 (MKX38_12750) | - | 2483366..2483506 (-) | 141 | Protein_2461 | phage holin family protein | - |
| MKX38_RS12755 (MKX38_12755) | - | 2483533..2483718 (-) | 186 | Protein_2462 | phage terminase large subunit | - |
| MKX38_RS12760 (MKX38_12760) | terS | 2483715..2484482 (-) | 768 | WP_317680594.1 | phage terminase small subunit | - |
| MKX38_RS12765 (MKX38_12765) | - | 2484664..2484750 (+) | 87 | WP_073991391.1 | YjcZ family sporulation protein | - |
| MKX38_RS12770 (MKX38_12770) | - | 2484791..2484883 (+) | 93 | WP_088326573.1 | YjcZ family sporulation protein | - |
| MKX38_RS12775 (MKX38_12775) | - | 2485071..2485286 (-) | 216 | WP_273796126.1 | CotD family spore coat protein | - |
| MKX38_RS12780 (MKX38_12780) | - | 2485848..2486303 (-) | 456 | WP_033884487.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| MKX38_RS12785 (MKX38_12785) | - | 2486395..2487132 (-) | 738 | WP_033884489.1 | hypothetical protein | - |
| MKX38_RS12790 (MKX38_12790) | - | 2487308..2487514 (-) | 207 | WP_033884491.1 | XtrA/YqaO family protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14951.97 Da Isoelectric Point: 5.1854
>NTDB_id=968321 MKX38_RS12665 WP_227057745.1 2467678..2468088(-) (nucA/comI) [Bacillus sp. FSL M8-0025]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGFDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGFDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=968321 MKX38_RS12665 WP_227057745.1 2467678..2468088(-) (nucA/comI) [Bacillus sp. FSL M8-0025]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTTTGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTTTGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
61.739 |
84.559 |
0.522 |