Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACK2VW_RS23340 Genome accession   NZ_CP176678
Coordinates   4614349..4615731 (-) Length   460 a.a.
NCBI ID   WP_001029696.1    Uniprot ID   -
Organism   Escherichia coli O121 strain GTVSS-020     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4609349..4620731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2VW_RS23325 (ACK2VW_23325) ettA 4611122..4612789 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  ACK2VW_RS23330 (ACK2VW_23330) nadS 4612950..4613057 (-) 108 Protein_4574 NadS family protein -
  ACK2VW_RS23335 (ACK2VW_23335) nadR 4613096..4614328 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACK2VW_RS23340 (ACK2VW_23340) radA/sms 4614349..4615731 (-) 1383 WP_001029696.1 DNA repair protein RadA Machinery gene
  ACK2VW_RS23345 (ACK2VW_23345) serB 4615780..4616748 (-) 969 WP_001132955.1 phosphoserine phosphatase -
  ACK2VW_RS23350 (ACK2VW_23350) ytjB 4616854..4617498 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  ACK2VW_RS23355 (ACK2VW_23355) lplA 4617526..4618542 (+) 1017 WP_000105889.1 lipoate--protein ligase LplA -
  ACK2VW_RS23360 (ACK2VW_23360) - 4618574..4618837 (+) 264 Protein_4580 helix-turn-helix domain-containing protein -
  ACK2VW_RS23365 (ACK2VW_23365) deoD 4618998..4619717 (-) 720 WP_000224877.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49413.96 Da        Isoelectric Point: 7.1936

>NTDB_id=968302 ACK2VW_RS23340 WP_001029696.1 4614349..4615731(-) (radA/sms) [Escherichia coli O121 strain GTVSS-020]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEA
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=968302 ACK2VW_RS23340 WP_001029696.1 4614349..4615731(-) (radA/sms) [Escherichia coli O121 strain GTVSS-020]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAACTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGTGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAG
TGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGCC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.608

99.348

0.463
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae R6

43.64

99.13

0.433
  radA Streptococcus pneumoniae TIGR4

43.64

99.13

0.433
  radA Streptococcus pneumoniae D39

43.64

99.13

0.433
  radA Streptococcus pneumoniae Rx1

43.64

99.13

0.433
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43

Multiple sequence alignment