Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MKY56_RS12950 Genome accession   NZ_CP150257
Coordinates   2489339..2490106 (-) Length   255 a.a.
NCBI ID   WP_076458122.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0054     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2484339..2495106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY56_RS12930 (MKY56_12930) - 2484513..2485325 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  MKY56_RS12935 (MKY56_12935) ccpN 2485351..2485989 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  MKY56_RS12940 (MKY56_12940) glyS 2486122..2488161 (-) 2040 WP_076458121.1 glycine--tRNA ligase subunit beta -
  MKY56_RS12945 (MKY56_12945) glyQ 2488154..2489041 (-) 888 WP_076458153.1 glycine--tRNA ligase subunit alpha -
  MKY56_RS12950 (MKY56_12950) recO 2489339..2490106 (-) 768 WP_076458122.1 DNA repair protein RecO Machinery gene
  MKY56_RS12955 (MKY56_12955) - 2490143..2490286 (-) 144 WP_003230053.1 YqzL family protein -
  MKY56_RS12960 (MKY56_12960) era 2490434..2491339 (-) 906 WP_003230051.1 GTPase Era -
  MKY56_RS12965 (MKY56_12965) - 2491320..2491730 (-) 411 WP_032722130.1 cytidine deaminase -
  MKY56_RS12970 (MKY56_12970) - 2491849..2492220 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  MKY56_RS12975 (MKY56_12975) ybeY 2492201..2492674 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  MKY56_RS12980 (MKY56_12980) pgpH 2492675..2494810 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=968238 MKY56_RS12950 WP_076458122.1 2489339..2490106(-) (recO) [Bacillus sp. FSL M8-0054]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRVPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=968238 MKY56_RS12950 WP_076458122.1 2489339..2490106(-) (recO) [Bacillus sp. FSL M8-0054]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAAATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAGGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGGTACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment