Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKX71_RS01950 Genome accession   NZ_CP150256
Coordinates   367755..368204 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus sp. FSL M8-0071     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 362755..373204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX71_RS01915 (MKX71_01915) - 362910..363590 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  MKX71_RS01920 (MKX71_01920) - 363600..364463 (-) 864 WP_080528904.1 transporter substrate-binding domain-containing protein -
  MKX71_RS01925 (MKX71_01925) - 364679..364821 (+) 143 Protein_335 hypothetical protein -
  MKX71_RS01930 (MKX71_01930) - 364840..365293 (+) 454 Protein_336 RDD family protein -
  MKX71_RS01935 (MKX71_01935) - 365375..365707 (+) 333 WP_015252849.1 YckD family protein -
  MKX71_RS01940 (MKX71_01940) bglC 365860..367293 (+) 1434 WP_213380010.1 family 1 glycosylhydrolase -
  MKX71_RS01945 (MKX71_01945) nin/comJ 367330..367728 (-) 399 WP_032722902.1 DNA-entry nuclease inhibitor Regulator
  MKX71_RS01950 (MKX71_01950) nucA/comI 367755..368204 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  MKX71_RS01955 (MKX71_01955) tlpC 368372..370093 (-) 1722 WP_213420221.1 methyl-accepting chemotaxis protein -
  MKX71_RS01960 (MKX71_01960) hxlB 370204..370761 (-) 558 WP_339231679.1 6-phospho-3-hexuloisomerase -
  MKX71_RS01965 (MKX71_01965) hxlA 370767..371399 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  MKX71_RS01970 (MKX71_01970) hxlR 371631..371993 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=968117 MKX71_RS01950 WP_003234608.1 367755..368204(-) (nucA/comI) [Bacillus sp. FSL M8-0071]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=968117 MKX71_RS01950 WP_003234608.1 367755..368204(-) (nucA/comI) [Bacillus sp. FSL M8-0071]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTCTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment