Detailed information NTG0968115

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACK2V6_RS01245
• Genome accession: NZ_CP176657
• Coordinates: 265911..268115 (-)
• Length: 734 a.a.
• NCBI ID: WP_005481427.1
• Uniprot ID: Q87T17
• Organism: Vibrio parahaemolyticus strain GTVSS-032
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 260911..273115

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK2V6_RS01225 (ACK2V6_01225)	hslU	261778..263109 (-)	1332	WP_005457198.1	HslU--HslV peptidase ATPase subunit	-
ACK2V6_RS01230 (ACK2V6_01230)	hslV	263133..263684 (-)	552	WP_005455873.1	ATP-dependent protease subunit HslV	-
ACK2V6_RS01235 (ACK2V6_01235)	ftsN	263849..264394 (-)	546	WP_005481405.1	cell division protein FtsN	-
ACK2V6_RS01240 (ACK2V6_01240)	cytR	264571..265578 (-)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
ACK2V6_RS01245 (ACK2V6_01245)	priA	265911..268115 (-)	2205	WP_005481427.1	primosomal protein N'	Machinery gene
ACK2V6_RS01250 (ACK2V6_01250)	rpmE	268410..268631 (+)	222	WP_005457203.1	50S ribosomal protein L31	-
ACK2V6_RS01255 (ACK2V6_01255)	rpsJ	269089..269400 (+)	312	WP_004410492.1	30S ribosomal protein S10	-
ACK2V6_RS01260 (ACK2V6_01260)	rplC	269415..270044 (+)	630	WP_005456132.1	50S ribosomal protein L3	-
ACK2V6_RS01265 (ACK2V6_01265)	rplD	270062..270664 (+)	603	WP_005379556.1	50S ribosomal protein L4	-
ACK2V6_RS01270 (ACK2V6_01270)	rplW	270661..270963 (+)	303	WP_004398471.1	50S ribosomal protein L23	-
ACK2V6_RS01275 (ACK2V6_01275)	rplB	270979..271803 (+)	825	WP_005489461.1	50S ribosomal protein L2	-
ACK2V6_RS01280 (ACK2V6_01280)	rpsS	271825..272103 (+)	279	WP_004394525.1	30S ribosomal protein S19	-
ACK2V6_RS01285 (ACK2V6_01285)	rplV	272114..272446 (+)	333	WP_005383164.1	50S ribosomal protein L22	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82271.83 Da
• Isoelectric Point: 8.5714

>NTDB_id=968115 ACK2V6_RS01245 WP_005481427.1 265911..268115(-) (priA) [Vibrio parahaemolyticus strain GTVSS-032]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWPEKLYS
LLIWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAIKQQKVLQMLVNGALPHQEFVDQ
EITSTVLKSLEDKGWIERIEKKPAITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTSNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLIQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=968115 ACK2V6_RS01245 WP_005481427.1 265911..268115(-) (priA) [Vibrio parahaemolyticus strain GTVSS-032]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGCCTGAAAAGCTTTATTCG
TTGCTGATTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGATCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAACCTCAACTGTACTAAAGTCTTTAGAAGATAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGCAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGCGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAACGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGATCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCCCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCTACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTTCTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCGCAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTGGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGAGATAGCACGCGCCGCAAAGGCAGTTTAGAAGATGCGCTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTGATAGGCACTCAAATGTTGGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTCGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAACACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCTCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAA
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• DEAD/DEAH box helicase (DEAD) (220-365)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

3D structure

• AlphaFold DB: Q87T17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.649	100	0.44