Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MKZ05_RS11330 Genome accession   NZ_CP150255
Coordinates   2257473..2258543 (-) Length   356 a.a.
NCBI ID   WP_249118252.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0277     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2252473..2263543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ05_RS11280 (MKZ05_11280) - 2252566..2253138 (-) 573 WP_003217306.1 signal peptidase I -
  MKZ05_RS11285 (MKZ05_11285) tapA 2253190..2253717 (-) 528 WP_212045914.1 amyloid fiber anchoring/assembly protein TapA -
  MKZ05_RS11290 (MKZ05_11290) - 2254017..2254295 (+) 279 WP_249118272.1 DUF3889 domain-containing protein -
  MKZ05_RS11295 (MKZ05_11295) - 2254329..2254523 (-) 195 WP_034620826.1 YqzE family protein -
  MKZ05_RS11300 (MKZ05_11300) comGG 2254583..2254966 (-) 384 WP_212045913.1 competence type IV pilus minor pilin ComGG -
  MKZ05_RS11305 (MKZ05_11305) comGF 2254963..2255463 (-) 501 WP_212045912.1 competence type IV pilus minor pilin ComGF -
  MKZ05_RS11310 (MKZ05_11310) comGE 2255402..2255719 (-) 318 WP_003217390.1 competence type IV pilus minor pilin ComGE -
  MKZ05_RS11315 (MKZ05_11315) comGD 2255703..2256149 (-) 447 WP_212045911.1 competence type IV pilus minor pilin ComGD -
  MKZ05_RS11320 (MKZ05_11320) comGC 2256142..2256435 (-) 294 WP_212045910.1 competence type IV pilus major pilin ComGC Machinery gene
  MKZ05_RS11325 (MKZ05_11325) comGB 2256452..2257492 (-) 1041 WP_212045909.1 competence type IV pilus assembly protein ComGB -
  MKZ05_RS11330 (MKZ05_11330) comGA 2257473..2258543 (-) 1071 WP_249118252.1 competence type IV pilus ATPase ComGA Machinery gene
  MKZ05_RS11335 (MKZ05_11335) - 2258721..2259593 (-) 873 WP_003217438.1 STAS domain-containing protein -
  MKZ05_RS11345 (MKZ05_11345) - 2259817..2260197 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKZ05_RS11350 (MKZ05_11350) - 2260410..2260652 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MKZ05_RS11355 (MKZ05_11355) - 2260897..2261535 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  MKZ05_RS11360 (MKZ05_11360) - 2261693..2261866 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MKZ05_RS11365 (MKZ05_11365) - 2261892..2262206 (-) 315 WP_003217504.1 MTH1187 family thiamine-binding protein -
  MKZ05_RS11370 (MKZ05_11370) - 2262285..2263538 (-) 1254 WP_003217413.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40361.76 Da        Isoelectric Point: 9.2487

>NTDB_id=968085 MKZ05_RS11330 WP_249118252.1 2257473..2258543(-) (comGA) [Bacillus sp. FSL M8-0277]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLHRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=968085 MKZ05_RS11330 WP_249118252.1 2257473..2258543(-) (comGA) [Bacillus sp. FSL M8-0277]
TTGTATGGGATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGGGGTCAGGTGTTTATTCGGATGTCTACTCTACCAACTGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACACCTGTCACTATTTCCGAAAGCATCCACCAGGTTATTATCCTTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACAGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCGGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTTGGTGAGTGTATCAAGGAAGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCATAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment