Detailed information NTG0968028

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACK2V4_RS01310
• Genome accession: NZ_CP176646
• Coordinates: 278152..280356 (-)
• Length: 734 a.a.
• NCBI ID: WP_029805229.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain GTVSS-031
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 273152..285356

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK2V4_RS01290 (ACK2V4_01290)	hslU	274019..275350 (-)	1332	WP_025441237.1	HslU--HslV peptidase ATPase subunit	-
ACK2V4_RS01295 (ACK2V4_01295)	hslV	275374..275925 (-)	552	WP_005455873.1	ATP-dependent protease subunit HslV	-
ACK2V4_RS01300 (ACK2V4_01300)	ftsN	276091..276636 (-)	546	WP_005481405.1	cell division protein FtsN	-
ACK2V4_RS01305 (ACK2V4_01305)	cytR	276812..277819 (-)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
ACK2V4_RS01310 (ACK2V4_01310)	priA	278152..280356 (-)	2205	WP_029805229.1	primosomal protein N'	Machinery gene
ACK2V4_RS01315 (ACK2V4_01315)	rpmE	280651..280872 (+)	222	WP_005457203.1	50S ribosomal protein L31	-
ACK2V4_RS01320 (ACK2V4_01320)	rpsJ	281330..281641 (+)	312	WP_004410492.1	30S ribosomal protein S10	-
ACK2V4_RS01325 (ACK2V4_01325)	rplC	281656..282285 (+)	630	WP_005456132.1	50S ribosomal protein L3	-
ACK2V4_RS01330 (ACK2V4_01330)	rplD	282303..282905 (+)	603	WP_005379556.1	50S ribosomal protein L4	-
ACK2V4_RS01335 (ACK2V4_01335)	rplW	282902..283204 (+)	303	WP_004398471.1	50S ribosomal protein L23	-
ACK2V4_RS01340 (ACK2V4_01340)	rplB	283220..284044 (+)	825	WP_005489461.1	50S ribosomal protein L2	-
ACK2V4_RS01345 (ACK2V4_01345)	rpsS	284066..284344 (+)	279	WP_004394525.1	30S ribosomal protein S19	-
ACK2V4_RS01350 (ACK2V4_01350)	rplV	284355..284687 (+)	333	WP_005383164.1	50S ribosomal protein L22	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82299.93 Da
• Isoelectric Point: 8.7811

>NTDB_id=968028 ACK2V4_RS01310 WP_029805229.1 278152..280356(-) (priA) [Vibrio parahaemolyticus strain GTVSS-031]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWPEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWRITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITKWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=968028 ACK2V4_RS01310 WP_029805229.1 278152..280356(-) (priA) [Vibrio parahaemolyticus strain GTVSS-031]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGTGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGCCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCGAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCAA
GTGGGGGCAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTTAACCTGATCAAA
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACTCGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAAACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGTAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCGCAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAATTGTTTCCCGAATACAA
AGCCATCCGAATAGACAGGGATAGCACGCGCCGCAAAGGCAGTTTAGAAGATGCGCTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTGATTGGCACTCAAATGTTAGCTAAGGGACACCATTTTCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGAGCAAGTAAGCCGGGGGAAGTGGTTTTACAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAAG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCGCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• DEAD/DEAH box helicase (DEAD) (220-365)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.419	100	0.436
priA	Bacillus subtilis subsp. subtilis str. 168	34.131	100	0.369