Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NYE53_RS12850 Genome accession   NZ_CP150251
Coordinates   2517302..2517712 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus sp. FSL R5-0523     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2512302..2522712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE53_RS12820 (NYE53_12820) - 2512389..2512529 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NYE53_RS12825 (NYE53_12825) - 2512835..2513566 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NYE53_RS12830 (NYE53_12830) cwlH 2513818..2514570 (-) 753 WP_339245015.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NYE53_RS12835 (NYE53_12835) - 2514757..2515383 (+) 627 WP_339245018.1 TVP38/TMEM64 family protein -
  NYE53_RS12840 (NYE53_12840) gnd 2515402..2516295 (-) 894 WP_339245021.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NYE53_RS12845 (NYE53_12845) - 2516547..2517269 (+) 723 WP_339245024.1 DUF308 domain-containing protein -
  NYE53_RS12850 (NYE53_12850) nucA/comI 2517302..2517712 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NYE53_RS12855 (NYE53_12855) sigK 2517908..2518636 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NYE53_RS12860 (NYE53_12860) - 2518636..2518752 (+) 117 WP_339245027.1 hypothetical protein -
  NYE53_RS12865 (NYE53_12865) - 2518718..2518914 (-) 197 Protein_2484 recombinase family protein -
  NYE53_RS12870 (NYE53_12870) - 2518872..2519036 (-) 165 WP_339245030.1 hypothetical protein -
  NYE53_RS12875 (NYE53_12875) psiE 2519581..2519997 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  NYE53_RS12880 (NYE53_12880) yrkQ 2520043..2521341 (-) 1299 WP_339245033.1 two-component system sensor histidine kinase YrkQ -
  NYE53_RS12885 (NYE53_12885) yrkP 2521328..2522023 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=967814 NYE53_RS12850 WP_009967785.1 2517302..2517712(-) (nucA/comI) [Bacillus sp. FSL R5-0523]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=967814 NYE53_RS12850 WP_009967785.1 2517302..2517712(-) (nucA/comI) [Bacillus sp. FSL R5-0523]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment