Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MKY43_RS13270 Genome accession   NZ_CP150249
Coordinates   2564914..2565543 (-) Length   209 a.a.
NCBI ID   WP_003183693.1    Uniprot ID   Q8VQ77
Organism   Bacillus sp. FSL M8-0133     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2559914..2570543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY43_RS13245 (MKY43_13245) rpsT 2560128..2560394 (+) 267 WP_003183682.1 30S ribosomal protein S20 -
  MKY43_RS13250 (MKY43_13250) holA 2560456..2561499 (-) 1044 WP_009327866.1 DNA polymerase III subunit delta -
  MKY43_RS13255 (MKY43_13255) - 2561729..2561860 (+) 132 WP_003183686.1 YqzM family protein -
  MKY43_RS13260 (MKY43_13260) - 2561885..2564272 (-) 2388 WP_061565979.1 DNA internalization-related competence protein ComEC/Rec2 -
  MKY43_RS13265 (MKY43_13265) - 2564276..2564845 (-) 570 WP_003183690.1 ComE operon protein 2 -
  MKY43_RS13270 (MKY43_13270) comEA 2564914..2565543 (-) 630 WP_003183693.1 helix-hairpin-helix domain-containing protein Machinery gene
  MKY43_RS13275 (MKY43_13275) comER 2565627..2566448 (+) 822 WP_003183696.1 late competence protein ComER -
  MKY43_RS13280 (MKY43_13280) - 2566518..2567273 (-) 756 WP_003183697.1 class I SAM-dependent methyltransferase -
  MKY43_RS13285 (MKY43_13285) rsfS 2567270..2567626 (-) 357 WP_003183699.1 ribosome silencing factor -
  MKY43_RS13290 (MKY43_13290) yqeK 2567640..2568203 (-) 564 WP_003183701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MKY43_RS13295 (MKY43_13295) - 2568193..2568762 (-) 570 WP_003183702.1 nicotinate-nucleotide adenylyltransferase -
  MKY43_RS13300 (MKY43_13300) yhbY 2568781..2569071 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  MKY43_RS13305 (MKY43_13305) aroE 2569065..2569901 (-) 837 WP_003183706.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22468.42 Da        Isoelectric Point: 5.1890

>NTDB_id=967707 MKY43_RS13270 WP_003183693.1 2564914..2565543(-) (comEA) [Bacillus sp. FSL M8-0133]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTYDKKEEVKREKSAGKEAVIIDLKGAVKNPGV
YQMKEGDRVHDVLKKAGGTEKKADQKQINLAAVLQDGMVVYIPFEGEEAADSFSKAGSRADGASVDIVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFHTVEDITNVSGIGEKSFERIKSAITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=967707 MKY43_RS13270 WP_003183693.1 2564914..2565543(-) (comEA) [Bacillus sp. FSL M8-0133]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCTGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCCGAGGAGGCTTCTGCACAGACGTATGACAAGAAGG
AGGAGGTGAAACGGGAAAAAAGCGCGGGAAAAGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAAGAGGGAGACAGGGTCCACGATGTATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGATCAAAAGCA
AATTAACCTTGCAGCCGTTTTGCAGGACGGTATGGTGGTTTACATTCCATTTGAGGGGGAAGAGGCTGCTGATTCTTTCT
CGAAAGCGGGTTCAAGGGCAGATGGCGCTTCCGTCGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCCGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGCGAGGAGAACGGACCGTTTCACACGGTAGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTGCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(147-207)

SLBB domain (SLBB)

(69-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526

Multiple sequence alignment