Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MKY43_RS12765 Genome accession   NZ_CP150249
Coordinates   2473228..2474166 (-) Length   312 a.a.
NCBI ID   WP_061565980.1    Uniprot ID   A0A8B5Y947
Organism   Bacillus sp. FSL M8-0133     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2468228..2479166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY43_RS12720 (MKY43_12720) tapA 2468728..2469456 (-) 729 WP_003183451.1 amyloid fiber anchoring/assembly protein TapA -
  MKY43_RS12725 (MKY43_12725) - 2469766..2470053 (+) 288 WP_223307118.1 YqzG/YhdC family protein -
  MKY43_RS12730 (MKY43_12730) - 2470077..2470259 (-) 183 WP_003183456.1 YqzE family protein -
  MKY43_RS12735 (MKY43_12735) comGG 2470348..2470713 (-) 366 WP_003183459.1 competence type IV pilus minor pilin ComGG -
  MKY43_RS12740 (MKY43_12740) comGF 2470726..2471214 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  MKY43_RS12745 (MKY43_12745) comGE 2471123..2471470 (-) 348 WP_009327907.1 competence type IV pilus minor pilin ComGE -
  MKY43_RS12750 (MKY43_12750) comGD 2471454..2471891 (-) 438 WP_009327905.1 competence type IV pilus minor pilin ComGD -
  MKY43_RS12755 (MKY43_12755) comGC 2471897..2472190 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  MKY43_RS12760 (MKY43_12760) comGB 2472204..2473238 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  MKY43_RS12765 (MKY43_12765) comGA 2473228..2474166 (-) 939 WP_061565980.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  MKY43_RS12770 (MKY43_12770) - 2474323..2475162 (-) 840 WP_003183480.1 STAS domain-containing protein -
  MKY43_RS12780 (MKY43_12780) - 2475404..2475781 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKY43_RS12785 (MKY43_12785) - 2475990..2476235 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  MKY43_RS12790 (MKY43_12790) - 2476273..2476911 (-) 639 WP_003183485.1 MBL fold metallo-hydrolase -
  MKY43_RS12795 (MKY43_12795) - 2477067..2477240 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  MKY43_RS12800 (MKY43_12800) - 2477301..2477615 (-) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -
  MKY43_RS12805 (MKY43_12805) - 2477631..2478740 (-) 1110 WP_003183488.1 hypothetical protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35224.00 Da        Isoelectric Point: 9.1258

>NTDB_id=967704 MKY43_RS12765 WP_061565980.1 2473228..2474166(-) (comGA) [Bacillus sp. FSL M8-0133]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDGLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINREPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIDY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVSAQRLVDIVCPFCG
DSCTLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRCGGIKTDTLKMLIRKGIALGYLPSKTYERWIGHED

Nucleotide


Download         Length: 939 bp        

>NTDB_id=967704 MKY43_RS12765 WP_061565980.1 2473228..2474166(-) (comGA) [Bacillus sp. FSL M8-0133]
TTGTACACGATCGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTACGCTTCAGGATTGATGACGAACTGTTTCAAAAAGACGGATTAACGAGAAATGAGTGCT
CTAGACTCATTTCTCACTTTAAATTTCTTTCTTCGATGGACATTGGCGAGAGACGGCTGCCGCAAAGCGGGGCTTTAACT
CTTTATATCAACCGTGAGCCCGTTCATTTAAGGATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTCCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATGCTTTTCACCGGCCCGACAGGCTCGGGAAAAACGACAACACTCTATTCTTTAATTGACTAC
GCCAAGCGGCATTTTAAGCGAAATATCATTACGTTGGAAGACCCGGTCGAATCAAGAAGCGAAAACATTTTGCAAGTACA
AGTGAACGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTGCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGCGCCCAGCGTCTTGTCGATATCGTCTGTCCGTTTTGCGGC
GACAGCTGCACTTTGTATTGCAGATTGTCGAGACCAGTTAGAAGAGCAAGCGTCTTTGAACTTTTGTACGGAAAAAGCCT
TAATCTCTGCATCGAAGAAGCAAAAGGCAGATGCGGTGGCATCAAAACAGACACCTTAAAAATGCTGATCCGCAAGGGAA
TAGCACTCGGATATCTGCCGTCGAAAACTTATGAACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B5Y947

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.757

100

0.667

Multiple sequence alignment