Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MKY61_RS20530 Genome accession   NZ_CP150247
Coordinates   3860478..3861872 (-) Length   464 a.a.
NCBI ID   WP_003185720.1    Uniprot ID   A0AB37GV19
Organism   Bacillus sp. FSL M8-0315     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3855478..3866872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY61_RS20495 (MKY61_20495) flgL 3855668..3856579 (-) 912 WP_071583859.1 flagellar hook-associated protein FlgL -
  MKY61_RS20500 (MKY61_20500) flgK 3856590..3858113 (-) 1524 WP_071583858.1 flagellar hook-associated protein FlgK -
  MKY61_RS20505 (MKY61_20505) - 3858129..3858605 (-) 477 WP_003185711.1 flagellar protein FlgN -
  MKY61_RS20510 (MKY61_20510) flgM 3858624..3858890 (-) 267 WP_003185713.1 flagellar biosynthesis anti-sigma factor FlgM -
  MKY61_RS20515 (MKY61_20515) - 3858970..3859389 (-) 420 WP_003185715.1 TIGR03826 family flagellar region protein -
  MKY61_RS20520 (MKY61_20520) comFC 3859448..3860182 (-) 735 WP_011198352.1 amidophosphoribosyltransferase Machinery gene
  MKY61_RS20525 (MKY61_20525) - 3860139..3860423 (-) 285 WP_003185718.1 competence protein ComFB -
  MKY61_RS20530 (MKY61_20530) comFA 3860478..3861872 (-) 1395 WP_003185720.1 DEAD/DEAH box helicase Machinery gene
  MKY61_RS20535 (MKY61_20535) - 3861994..3862836 (-) 843 WP_003185726.1 DegV family protein -
  MKY61_RS20540 (MKY61_20540) degU 3862956..3863645 (-) 690 WP_003185730.1 two-component system response regulator DegU Regulator
  MKY61_RS20545 (MKY61_20545) degS 3863727..3864884 (-) 1158 WP_003185733.1 histidine kinase Regulator
  MKY61_RS20550 (MKY61_20550) - 3865098..3865736 (+) 639 WP_003185736.1 YigZ family protein -
  MKY61_RS20555 (MKY61_20555) - 3865751..3866824 (+) 1074 WP_017473894.1 LCP family protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52848.50 Da        Isoelectric Point: 9.9576

>NTDB_id=967608 MKY61_RS20530 WP_003185720.1 3860478..3861872(-) (comFA) [Bacillus sp. FSL M8-0315]
MIHIEQPAFYTRELRSCLEQRHLLKSELPFRESVVDWHIQEGLIKTEEGIKKTKKGFICLRCGQHERSFFARYPCYRCSK
CCVYCRSCVMMGRVSDCTPLLTWHMHVSHKWAPVHTEWKGALSAGQEKAAKSIINAIRRKEELLVWAVCGSGKTELLFQG
IEFALNNGLTVCIATPRTDVVLELAPRFRQAFPGVEIAALYGGSSDVGNLSPLIISTTHQLLRYKEAFDVIIIDEVDAFP
YSIDNTLQYAVKKSAKRQSAHIYLTATPSADMKKRAESGKLDTVRIPARFHRSPLPEPTLIWCGNWKKSLKRRKVPFRLK
KWLFKHQELQQPVFLFVPSVPVLKSVVSVLKKETFRAEGVYADDPDRNEKVNRFRESKLEVLVTTTILERGVTVKKAQVG
VLGAESAVFTESALVQMAGRAGRHPEHTDGDVCFFHYGRTKSMNAARRHIQNMNKMSKKEMLID

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=967608 MKY61_RS20530 WP_003185720.1 3860478..3861872(-) (comFA) [Bacillus sp. FSL M8-0315]
GTGATTCACATCGAACAACCGGCATTCTATACCCGTGAATTGCGGTCATGTTTGGAGCAGCGCCACCTTCTGAAAAGCGA
ACTTCCTTTTCGTGAATCCGTCGTTGATTGGCATATCCAAGAAGGCTTGATAAAAACGGAGGAAGGTATTAAAAAAACGA
AGAAAGGCTTTATTTGTTTGAGGTGCGGCCAGCACGAACGCTCATTTTTCGCCCGGTATCCTTGCTATCGGTGCAGTAAA
TGCTGCGTGTACTGCCGATCCTGCGTCATGATGGGCAGGGTGAGCGATTGTACGCCTCTGTTGACTTGGCACATGCATGT
CAGCCATAAATGGGCGCCTGTTCATACGGAGTGGAAAGGCGCGCTTTCAGCAGGTCAGGAGAAAGCGGCCAAATCCATTA
TCAATGCTATACGAAGAAAAGAAGAACTGTTGGTCTGGGCGGTTTGCGGGTCGGGGAAAACAGAACTTCTCTTTCAAGGG
ATCGAATTCGCGCTGAACAACGGCTTGACAGTATGTATCGCTACTCCGAGAACCGATGTCGTACTCGAGCTTGCTCCGCG
GTTTCGCCAAGCCTTTCCAGGGGTGGAAATTGCCGCTTTGTACGGAGGAAGCTCAGACGTTGGGAATCTCTCGCCGCTTA
TCATTTCAACTACCCACCAGCTGCTCCGCTATAAAGAAGCATTTGACGTGATCATCATAGATGAGGTGGATGCTTTTCCG
TATTCTATTGATAACACGCTGCAATACGCTGTTAAAAAATCGGCAAAACGGCAAAGCGCTCACATCTATTTAACTGCCAC
GCCTTCAGCAGATATGAAAAAAAGGGCTGAGAGCGGAAAGCTGGACACCGTCCGTATTCCCGCGAGATTCCACCGCAGCC
CCTTGCCTGAACCTACATTGATTTGGTGCGGAAATTGGAAAAAGAGTTTGAAACGAAGAAAAGTTCCTTTTCGCCTCAAA
AAATGGCTTTTTAAGCACCAAGAACTGCAGCAACCGGTTTTTTTATTCGTTCCCTCTGTCCCTGTTCTTAAATCTGTGGT
CAGTGTGCTGAAAAAGGAAACATTTCGCGCTGAAGGAGTTTATGCCGATGACCCTGACAGAAATGAAAAAGTGAACCGGT
TTAGGGAAAGTAAGCTTGAGGTGCTCGTGACAACAACGATTTTGGAAAGAGGAGTGACCGTCAAAAAAGCGCAGGTTGGA
GTATTGGGGGCAGAGTCGGCGGTTTTTACCGAAAGCGCCCTCGTCCAAATGGCGGGAAGAGCCGGCAGGCATCCCGAGCA
CACAGATGGAGACGTCTGTTTCTTTCATTACGGCAGGACAAAGTCGATGAATGCGGCACGACGTCATATTCAAAACATGA
ATAAAATGTCTAAAAAGGAAATGTTGATTGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(122-269)

Helicase conserved C-terminal domain (Helicase_C)

(323-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

56.332

98.707

0.556

Multiple sequence alignment