Detailed information    

insolico Bioinformatically predicted

Overview


Name   rok   Type   Regulator
Locus tag   MKY61_RS10555 Genome accession   NZ_CP150247
Coordinates   2042895..2043371 (+) Length   158 a.a.
NCBI ID   WP_003182174.1    Uniprot ID   A0A1Y0YPP5
Organism   Bacillus sp. FSL M8-0315     
Function   repression of comK (predicted from homology)   
Competence regulation

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1990876..2045927 2042895..2043371 within 0


Gene organization within MGE regions


Location: 1990876..2045927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY61_RS10250 (MKY61_10250) - 1990876..1992021 (-) 1146 WP_071583919.1 site-specific integrase -
  MKY61_RS10255 (MKY61_10255) - 1992028..1992393 (-) 366 WP_071583954.1 ImmA/IrrE family metallo-endopeptidase -
  MKY61_RS10260 (MKY61_10260) - 1992402..1992830 (-) 429 WP_026587142.1 helix-turn-helix transcriptional regulator -
  MKY61_RS10265 (MKY61_10265) - 1993099..1993284 (+) 186 WP_026587143.1 helix-turn-helix transcriptional regulator -
  MKY61_RS10270 (MKY61_10270) - 1993290..1993559 (+) 270 WP_061578517.1 group-specific protein -
  MKY61_RS10275 (MKY61_10275) - 1993683..1993949 (+) 267 WP_009330095.1 YqaH family protein -
  MKY61_RS10280 (MKY61_10280) - 1994037..1994279 (+) 243 WP_011198322.1 hypothetical protein -
  MKY61_RS10285 (MKY61_10285) - 1994373..1994819 (+) 447 WP_069500487.1 hypothetical protein -
  MKY61_RS10290 (MKY61_10290) - 1994816..1995991 (+) 1176 WP_196770007.1 DUF2800 domain-containing protein -
  MKY61_RS10295 (MKY61_10295) - 1996023..1996562 (+) 540 WP_071583882.1 DUF2815 family protein -
  MKY61_RS10300 (MKY61_10300) - 1996651..1996896 (+) 246 WP_069500491.1 hypothetical protein -
  MKY61_RS10305 (MKY61_10305) - 1996902..1998845 (+) 1944 WP_069500492.1 DNA polymerase -
  MKY61_RS10310 (MKY61_10310) - 1998961..2001372 (+) 2412 WP_081329458.1 VapE domain-containing protein -
  MKY61_RS10315 (MKY61_10315) - 2001661..2001945 (+) 285 WP_069500915.1 VRR-NUC domain-containing protein -
  MKY61_RS10320 (MKY61_10320) - 2001935..2003290 (+) 1356 WP_069500496.1 DEAD/DEAH box helicase -
  MKY61_RS10325 (MKY61_10325) - 2003287..2003667 (+) 381 WP_071584222.1 ArpU family phage packaging/lysis transcriptional regulator -
  MKY61_RS10330 (MKY61_10330) cotD 2004408..2004632 (+) 225 WP_003185368.1 spore coat protein CotD -
  MKY61_RS10335 (MKY61_10335) - 2004886..2005560 (+) 675 WP_071583909.1 hypothetical protein -
  MKY61_RS10340 (MKY61_10340) - 2005636..2005944 (+) 309 WP_081364462.1 hypothetical protein -
  MKY61_RS10345 (MKY61_10345) - 2005971..2006345 (+) 375 WP_071583908.1 HNH endonuclease -
  MKY61_RS10350 (MKY61_10350) - 2006576..2007091 (+) 516 WP_071583907.1 phage terminase small subunit P27 family -
  MKY61_RS10355 (MKY61_10355) - 2007088..2008797 (+) 1710 WP_071583906.1 terminase TerL endonuclease subunit -
  MKY61_RS10360 (MKY61_10360) - 2008810..2009001 (+) 192 WP_035316082.1 DUF1056 family protein -
  MKY61_RS10365 (MKY61_10365) - 2009002..2010312 (+) 1311 WP_071583905.1 phage portal protein -
  MKY61_RS10370 (MKY61_10370) - 2010257..2010988 (+) 732 WP_071583904.1 head maturation protease, ClpP-related -
  MKY61_RS10375 (MKY61_10375) - 2011059..2012339 (+) 1281 WP_075646713.1 phage major capsid protein -
  MKY61_RS10380 (MKY61_10380) - 2012362..2012787 (+) 426 WP_065643430.1 collagen-like protein -
  MKY61_RS10385 (MKY61_10385) - 2012808..2013110 (+) 303 WP_003185351.1 head-tail connector protein -
  MKY61_RS10390 (MKY61_10390) - 2013100..2013408 (+) 309 WP_065643429.1 phage head closure protein -
  MKY61_RS10395 (MKY61_10395) - 2013408..2013805 (+) 398 Protein_2003 HK97-gp10 family putative phage morphogenesis protein -
  MKY61_RS10400 (MKY61_10400) - 2013802..2014185 (+) 384 WP_043054227.1 DUF3168 domain-containing protein -
  MKY61_RS10405 (MKY61_10405) - 2014200..2014817 (+) 618 WP_043054228.1 major tail protein -
  MKY61_RS10410 (MKY61_10410) - 2014871..2015236 (+) 366 WP_071583608.1 hypothetical protein -
  MKY61_RS10415 (MKY61_10415) - 2015445..2019914 (+) 4470 WP_095324191.1 phage tail tape measure protein -
  MKY61_RS10420 (MKY61_10420) - 2019914..2020750 (+) 837 WP_069500502.1 phage tail family protein -
  MKY61_RS10425 (MKY61_10425) - 2020763..2022475 (+) 1713 WP_071584250.1 phage tail protein -
  MKY61_RS10430 (MKY61_10430) - 2022512..2025157 (+) 2646 WP_071583773.1 peptidase G2 autoproteolytic cleavage domain-containing protein -
  MKY61_RS10435 (MKY61_10435) - 2025171..2026520 (+) 1350 WP_081364464.1 phage baseplate upper protein -
  MKY61_RS10440 (MKY61_10440) - 2026533..2026856 (+) 324 WP_069500506.1 hypothetical protein -
  MKY61_RS10445 (MKY61_10445) - 2026853..2027035 (+) 183 WP_039072971.1 XkdX family protein -
  MKY61_RS10450 (MKY61_10450) - 2027099..2027368 (+) 270 WP_069500916.1 hemolysin XhlA family protein -
  MKY61_RS10455 (MKY61_10455) - 2027384..2027647 (+) 264 WP_069500507.1 phage holin -
  MKY61_RS10460 (MKY61_10460) - 2027695..2028648 (+) 954 WP_069500508.1 glycoside hydrolase family 25 protein -
  MKY61_RS10465 (MKY61_10465) - 2028771..2029109 (-) 339 WP_069500509.1 hypothetical protein -
  MKY61_RS10470 (MKY61_10470) - 2029125..2030654 (-) 1530 WP_269195050.1 T7SS effector LXG polymorphic toxin -
  MKY61_RS10475 (MKY61_10475) - 2030850..2030993 (+) 144 WP_021837703.1 hypothetical protein -
  MKY61_RS10480 (MKY61_10480) - 2031248..2031478 (+) 231 WP_011198296.1 helix-turn-helix domain-containing protein -
  MKY61_RS10485 (MKY61_10485) - 2031502..2031869 (-) 368 Protein_2021 YolD-like family protein -
  MKY61_RS10490 (MKY61_10490) - 2031959..2032771 (-) 813 WP_035338318.1 hypothetical protein -
  MKY61_RS10495 (MKY61_10495) - 2033373..2033576 (+) 204 WP_003182148.1 hypothetical protein -
  MKY61_RS10500 (MKY61_10500) - 2034678..2035145 (+) 468 WP_003182152.1 DUF6323 family protein -
  MKY61_RS10505 (MKY61_10505) - 2035147..2036583 (+) 1437 WP_003182154.1 DUF6179 domain-containing protein -
  MKY61_RS10510 (MKY61_10510) - 2036710..2037027 (-) 318 WP_003182156.1 hypothetical protein -
  MKY61_RS10515 (MKY61_10515) - 2037380..2037808 (+) 429 WP_003182158.1 HIT domain-containing protein -
  MKY61_RS10520 (MKY61_10520) - 2038452..2038754 (+) 303 WP_003182160.1 hypothetical protein -
  MKY61_RS10525 (MKY61_10525) - 2038987..2039964 (+) 978 WP_071583709.1 alpha/beta hydrolase family protein -
  MKY61_RS10530 (MKY61_10530) - 2040027..2040269 (+) 243 WP_003182164.1 hypothetical protein -
  MKY61_RS10535 (MKY61_10535) - 2040322..2040801 (+) 480 WP_003182166.1 hypothetical protein -
  MKY61_RS10540 (MKY61_10540) - 2040943..2041539 (+) 597 WP_003182167.1 class I SAM-dependent methyltransferase -
  MKY61_RS10545 (MKY61_10545) - 2041646..2042125 (+) 480 WP_003182169.1 YdhH/YoaO family protein -
  MKY61_RS10550 (MKY61_10550) - 2042159..2042734 (+) 576 WP_071583710.1 GNAT family N-acetyltransferase -
  MKY61_RS10555 (MKY61_10555) rok 2042895..2043371 (+) 477 WP_003182174.1 hypothetical protein Regulator
  MKY61_RS10560 (MKY61_10560) - 2043490..2043663 (+) 174 WP_003182176.1 hypothetical protein -
  MKY61_RS10565 (MKY61_10565) - 2044012..2044632 (-) 621 WP_003182178.1 hypothetical protein -
  MKY61_RS10570 (MKY61_10570) - 2045343..2045927 (+) 585 WP_009328246.1 TetR family transcriptional regulator -

Sequence


Protein


Download         Length: 158 a.a.        Molecular weight: 18302.15 Da        Isoelectric Point: 10.1366

>NTDB_id=967579 MKY61_RS10555 WP_003182174.1 2042895..2043371(+) (rok) [Bacillus sp. FSL M8-0315]
MFNEREALRLRLEQLGDAEIQVMRELRKEREGIYSKLRELDRESEPAINKKSSLIDLASAAAEELKQSQHAADKHPPIKT
PSHTIFQSKTTIQREAAINILSRYEEGLKGIKLKTEIEKETGFPIKNMTTFMKSLMKHRPDIKKPARGHYILHKEKDL

Nucleotide


Download         Length: 477 bp        

>NTDB_id=967579 MKY61_RS10555 WP_003182174.1 2042895..2043371(+) (rok) [Bacillus sp. FSL M8-0315]
ATGTTCAACGAAAGAGAAGCGTTGCGTTTGAGACTTGAACAGCTAGGTGATGCGGAGATCCAGGTGATGCGGGAATTGCG
AAAAGAACGAGAGGGCATTTATTCGAAGCTGCGTGAATTGGATCGGGAGAGTGAGCCTGCAATTAACAAAAAATCTTCAT
TAATCGACTTGGCAAGCGCTGCAGCAGAGGAATTAAAACAATCCCAGCATGCCGCTGACAAACATCCGCCTATCAAGACC
CCATCTCATACTATTTTCCAATCAAAAACAACCATACAGCGTGAGGCTGCCATAAACATTTTAAGCCGCTATGAAGAGGG
CTTGAAAGGCATTAAGCTCAAAACTGAAATCGAGAAAGAAACCGGCTTTCCGATTAAAAACATGACGACATTTATGAAGA
GCTTAATGAAACATCGCCCAGATATTAAAAAGCCTGCCCGCGGCCATTATATTTTGCATAAAGAAAAAGACCTTTGA

Domains



No domain identified.



Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1Y0YPP5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rok Bacillus subtilis subsp. subtilis str. 168

45.055

100

0.519


Multiple sequence alignment