Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ACKZ7S_RS04755 Genome accession   NZ_CP176565
Coordinates   950875..954528 (+) Length   1217 a.a.
NCBI ID   WP_162636060.1    Uniprot ID   -
Organism   Staphylococcus aureus strain V0353     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 945875..959528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKZ7S_RS04740 (ACKZ7S_04740) lepB 946123..946647 (+) 525 WP_162636058.1 signal peptidase I -
  ACKZ7S_RS04745 (ACKZ7S_04745) lepB 946663..947238 (+) 576 WP_000711897.1 signal peptidase I -
  ACKZ7S_RS04750 (ACKZ7S_04750) addB 947398..950874 (+) 3477 WP_162636059.1 helicase-exonuclease AddAB subunit AddB -
  ACKZ7S_RS04755 (ACKZ7S_04755) addA 950875..954528 (+) 3654 WP_162636060.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACKZ7S_RS04760 (ACKZ7S_04760) - 954694..955596 (+) 903 WP_000670752.1 fumarylacetoacetate hydrolase family protein -
  ACKZ7S_RS04765 (ACKZ7S_04765) - 955923..956312 (+) 390 WP_000902814.1 YisL family protein -
  ACKZ7S_RS04770 (ACKZ7S_04770) - 956483..957799 (-) 1317 WP_001124497.1 CoA-disulfide reductase -
  ACKZ7S_RS04775 (ACKZ7S_04775) - 957851..958675 (-) 825 WP_001182436.1 Cof-type HAD-IIB family hydrolase -
  ACKZ7S_RS04780 (ACKZ7S_04780) - 958789..959097 (+) 309 WP_000390576.1 metal-sulfur cluster assembly factor -

Sequence

Protein


Download         Length: 1217 a.a.        Molecular weight: 141228.83 Da        Isoelectric Point: 4.9264

>NTDB_id=967574 ACKZ7S_RS04755 WP_162636060.1 950875..954528(+) (addA) [Staphylococcus aureus strain V0353]
MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRI
QEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFITAAYDALNKAYDLFS
MMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGHEFGARLPNVTAKIKEVNEMMVDALEDAKLQYKKYKSLIDKVKSD
YFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILV
DEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTIDGDGTGRRIDLSQNFRSRKEVLS
TTNYIFKHMMDEQVGEVKYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMK
TGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQF
KEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIG
GRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKD
FNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLE
LEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQIEDSSRPNVNILIVYFEDVSTETILDNDEY
RSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQ
GKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSELYSIIAEAEQVYRELP
FVVNQALVDQLPQGDEDASIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILN
KEVKGYLYFFKFGTLQL

Nucleotide


Download         Length: 3654 bp        

>NTDB_id=967574 ACKZ7S_RS04755 WP_162636060.1 950875..954528(+) (addA) [Staphylococcus aureus strain V0353]
ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATCTACGCCACTGGACAAGATGT
ACTTGTTGCAGCTGCGGCAGGTTCAGGTAAAACAGCTGTACTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCA
TTGATGTCGATCGACTTTTAGTCGTTACGTTTACAAACTTAAGTGCACGTGAAATGAAGCATCGTGTAGACCAACGAATT
CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGCATCAAAATTCATCAAGCACAAATATCTAC
ACTTCATAGTTTTTGCTTGAAATTAATTCAACAGCATTATGATGTATTAAATATTGACCCGAACTTTAGAACGAGCAGTG
AAGCTGAAAATATTTTATTATTAGAACAAACGATAGATGAAGTCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
ATTGAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAG
CGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTA
TTCAACTACTAACAGACTTATCTAAAGTATTTATCACAGCTGCATATGATGCTTTAAATAAGGCGTATGATTTGTTTAGT
ATGATGGATGGCGTCGATAAACATTTAGCTGTTATAGAAGATGAACGACGTTTAATGGGGCGTGTTTTAGAAGGTGGCTT
TATTGATATACCTTATTTAACTGGTCACGAATTTGGCGCGCGTTTACCTAATGTAACAGCGAAAATTAAAGAAGTAAATG
AAATGATGGTCGATGCCTTAGAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTTAAGAGTGAT
TACTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAATTGGCGCCACGAGTAAAGTACCTTGCGCGTATTGT
GAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGCAAAAATATTTTGGATTTTTCTGATTATGAACATTTTGCAT
TACAAATTTTAACTAATGAGGATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACACTTCCAAGAAATATTGGTC
GATGAGTATCAAGATACGAACCGAGTTCAAGAGAAAATATTATCTTGTATCAAAACGGGTGATGAACATAATGGTAATTT
ATTTATGGTTGGAGATGTTAAGCAATCCATTTATAAATTTAGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAAC
GCTTTACTATAGATGGAGATGGCACTGGACGTCGAATTGATTTGTCGCAAAACTTCCGTTCACGTAAAGAAGTACTGTCA
ACGACAAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTA
TGGTGCACCATATGATGAATCAGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAA
CTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAA
ACAGGAAGCTATAGAAGTGCGACATACAAGGATATCGTTATTCTAGAACGCAGCTTTGGACAAGCTCGCAATTTACAACA
AGCCTTTAAAAATGAAGATATTCCATTCCATGTTAATAGTCGTGAAGGATACTTTGAACAAACAGAAGTCCGTTTAGTAT
TATCATTTTTAAGAGCGATAGATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGTTCCGTTATTTATCAGTTC
AAAGAAGACGAATTAGCTCAAATTAGAATATTGAGTCCAAATGATGACTACTTCTATCAATCGATTGTAAATTACATTAA
TGACGAAGCAGCAGATGCTATTTTAGTTGATAAATTAAAAATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTA
AAGATCATCCGGTGTATCAGTTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
GGACGTGGACGACGTGCAAATCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAATTCAAGTTTTAGAGGTTTATA
TCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGCAAAGATTTTGGTGAGGAAAACGTAGTTGGTCCAAACGATA
ATGTCGTTAGAATGATGACAATTCATAGTAGTAAAGGTCTAGAGTTTCCGTTTGTCATTTATTCTGGATTGTCAAAAGAT
TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATGGATTATTTTGATGTGGATAA
AGAAATGGCATTTCCATCTTTAGCTTCGGTTGCATATAGAGCTGTTGCCGAAAAAGAACTTGTGTCAGAAGAAATGCGAT
TAGTCTATGTAGCATTAACAAGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCGTTACTAGAA
CTAGAGCAATTGTCTATTTCTGGTGAGCACATTGCTGTCAATGAACGATTAACTTCACCAAATCCGTTCCATCTTATTTA
TAGTATTTTATCTAAACATCAATCTGCGTCAATTCCAGATGATTTAAAATTTGAAAAAGATATAGCACAAATTGAAGATA
GTAGTCGTCCGAATGTAAATATTTTAATTGTGTACTTTGAAGATGTGTCTACAGAAACCATTTTAGATAATGATGAATAT
CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAAGATGTTAAAGCACAAATTAAACACCAACTTGATTATCA
ATATCCATATGTAAATGATACTAAAAAGCCCTCAAAACAATCTGTTTCTGAATTGAAAAGACAATATGAAACAGAAGAAA
GTGGCACAAGTTACGAACGAGTAAGGCAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTGATGCAACATTTACCATTCAAAAAAGAACGCATATC
TGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGATAAACATATTATCGAAGCAGATGCGAAAAAAGATATCCGTA
TGGATGAAATAATGACATTTATCAATAGTGAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
TTTGTAGTTAACCAAGCATTAGTTGACCAATTGCCACAAGGAGACGAAGACGCCTCAATTATTCAAGGTATGATTGACTT
AATCTTTGTTAAAGATGGTGTGCATTATTTTGTAGACTATAAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATG
AAGAAATTGGTACACAATTAAAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATTCTTAAT
AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTGTAG

Domains

Predicted by InterProScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1040-1210)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(12-455)

UvrD-like helicase C-terminal domain (UvrD_C)

(466-869)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

36.804

100

0.38

Multiple sequence alignment