Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKX44_RS02000 Genome accession   NZ_CP150246
Coordinates   368672..369118 (-) Length   148 a.a.
NCBI ID   WP_003178810.1    Uniprot ID   A0A8B5YEZ5
Organism   Bacillus sp. FSL M8-0359     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 363672..374118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX44_RS01975 (MKX44_01975) - 364279..365154 (-) 876 WP_003178801.1 transporter substrate-binding domain-containing protein -
  MKX44_RS01980 (MKX44_01980) - 365338..365889 (+) 552 WP_003178803.1 NAD(P)H-dependent oxidoreductase -
  MKX44_RS01985 (MKX44_01985) - 365986..366711 (+) 726 WP_003178804.1 AAA family ATPase -
  MKX44_RS01990 (MKX44_01990) - 366781..368217 (+) 1437 WP_003178806.1 family 1 glycosylhydrolase -
  MKX44_RS01995 (MKX44_01995) nin/comJ 368256..368654 (-) 399 WP_003178808.1 competence protein ComJ Regulator
  MKX44_RS02000 (MKX44_02000) nucA/comI 368672..369118 (-) 447 WP_003178810.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MKX44_RS02005 (MKX44_02005) - 369385..369588 (+) 204 WP_003178812.1 hypothetical protein -
  MKX44_RS02010 (MKX44_02010) - 369594..370397 (+) 804 WP_017473997.1 protein kinase -
  MKX44_RS02015 (MKX44_02015) - 370417..372026 (-) 1610 Protein_363 methyl-accepting chemotaxis protein -
  MKX44_RS02020 (MKX44_02020) - 372217..372957 (-) 741 WP_003178817.1 LXG domain-containing protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16224.12 Da        Isoelectric Point: 4.9954

>NTDB_id=967477 MKX44_RS02000 WP_003178810.1 368672..369118(-) (nucA/comI) [Bacillus sp. FSL M8-0359]
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHIRKAIKKGHSEICTIDRDGAD
ERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISPSDNRGAGSWVGNQVSDYPDGTKVLFKIN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=967477 MKX44_RS02000 WP_003178810.1 368672..369118(-) (nucA/comI) [Bacillus sp. FSL M8-0359]
ATGGACAAGATTAAATCTTTGCTGATCACCTTGCTCGCCGTCGCCGTCGTGATCATCGGTATTGTATCTGGCGACTTTTT
CACTTCAGAACACCAGCCTTCGGGCCGGCAAAACGGCTCAAACTACGATGAAGTGCTGATTTTTCCTTCGGACCGCTATC
CTGAAACAGGCGCACACATTCGAAAGGCTATCAAAAAAGGGCACTCCGAAATCTGTACGATCGACAGGGACGGAGCCGAC
GAACGCCGAAAGGATTCATTGAAAGATGTTCCTTCCAAATCAGGCTATGACCGCGACGAGTGGCCGATGGCCATGTGCGA
AGAAGGCGGAACAGGTGCTTCTGTCGAGTATATCAGCCCGTCTGATAACCGTGGAGCAGGATCTTGGGTGGGCAATCAGG
TAAGCGATTATCCGGACGGGACGAAAGTACTTTTCAAGATTAATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B5YEZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

64.626

99.324

0.642

Multiple sequence alignment